NO对损伤诱导的豌豆幼苗叶片抗氧化系统的影响  

Effects of Nitric Oxide on Wounding Induction of Pea Seedling Leaves Antioxidant System

作　　者：包敖民 刘艳[1] 马慧[1] 王发林[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《中国农学通报》2015年第25期60-65,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然基金项目"鲜切芽苗机械损伤防御反应形成中茉莉酸信号的产生机制"(31260403)

摘　　要：为了明确损伤后豌豆幼苗叶片内NO产生的途径及NO对其诱导的抗氧化系统的作用,以豌豆幼苗为试材,研究损伤胁迫下其内源NO含量、NR和NOS活性的变化,以及只有NO供体SNP处理和NO清除剂PTIO、NOS抑制剂L-NAME、NR抑制剂Na N3喷施后损伤处理过24 h时对豌豆幼苗叶片内H2O2和O2^-·含量变化及对抗氧化酶SOD、POD、CAT、APX等的活性影响。结果表明,损伤处理后0~60 h内豌豆幼苗叶片内NO含量呈双峰曲线;其中损伤处理早期（第1峰值）NO的主要来源是NOS酶促途径,而后期（第2峰值）NO的主要来源是NR酶促途径;损伤处理24 h豌豆幼苗叶片内NO含量最高,此时H2O2和O2^-·含量接近对照水平,而SOD、POD、CAT、APX等的活性显著高于对照,NO供体SNP处理时有类似的结果,NO清除剂PTIO处理后H2O2和O2^-·含量升高,SOD、POD、CAT、APX等的活性降低。以上研究结果表明,NO可能通过增强保护酶的活性来降低损伤诱导的膜脂过氧化程度。In order to definite in the wounding pea seedling leaves the produced way of NO, and NO on theantioxidant system induced by injury. In this experiment pea seedlings were used to study its endogenous NOcontention under wounding stress, the activity changed of NR and NOS activity, as well as the activityinfluenced to antioxidant enzymes SOD, POD, CAT and APX and the contention changed of H2O2 and O2^-· inthe leaves of pea seedlings after 24 hours by spraying processed with SNP（donor of NO）, PTIO（scavenger ofNO）, L- NAME（inhibitors of NOS） and Na N3（inhibitors of NR） respectively. Results showed that NOcontention in the pea seedling leaves presented a bimodal curve between 0 hour and 60 hours after thewounding treatment. The main source of NO was NOS enzymatic way when the earlier period of woundingprocesses（the first peak value）. But the main source of NO was NR enzymatic way when the later period ofwounding processes（the second peak value）; during the first 24 hours, pea seedling leaf NO contention reacheda highest, and the contention of H2O2 and O2^-· closed to the control level, while the activity of SOD, POD, CATand APX was higher than control significantly. When treated with SNP, it had a similar result. After thetreatment with PTIO, the contention of H2O2 and O2^-· increased, and the activity of SOD, POD, CAT and APXdecreased. Conclusion of the research results showed that NO might reduced the membrane lipid peroxidationinduced by wounding degree through enhancing the activity of protective enzyme.

关 键 词：豌豆幼苗 伤害 NO 活性氧 

分 类 号：S643.3[农业科学—蔬菜学]