不同浓度碳源和氮源对灰树花胞外多糖液体发酵产量的影响  

Effects of Different Concentrations of Carbon and Nitrogen Sources on Production of Grifola frondosa Extracellular Polysaccharides

作　　者：管博文 吴凡[2] 陈青君[1] 王丹[2] 师雨梦 张国庆[2] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院生物科学与工程学院,北京102206

出　　处：《中国农学通报》2015年第25期75-78,共4页Chinese Agricultural Science Bulletin

基　　金：科技成果转化提升计划"林下经济关键技术示范与转化(市级)"(PXM2014_014207_000079);"十二五"国家科技支撑计划项目(2012BAD14B09);北京市农委2014"菜篮子"新型生产经营主体科技能力提升工程(2014clz001);北京高等学校青年英才计划(YETP1714);北京市本科生科学研究计划

摘　　要：以灰树花(Grifola frondosa)BUAGF-02菌株为材料,研究葡萄糖和蛋白胨浓度对液体发酵产多糖的影响。研究以可溶性淀粉、葡萄糖、酵母提取物、蛋白胨等基础发酵培养基,碳源试验葡萄糖浓度分别为5、10、15、20、25 g/L,氮源试验蛋白胨浓度为1、1.5、2、2.5、3 g/L,25℃、170 r/min振荡培养10天,离心收集发酵液和菌丝体,采用醇沉法收集多糖,菌丝体和多糖烘干后称重。葡萄糖浓度在20 g/L时,胞外多糖产量最高,达到0.3 g/100 m L;其浓度25 g/L时菌丝体干重最高,为1.82 g/100 m L。蛋白胨浓度3 g/L时,胞外多糖产量最高,为0.2 g/100 m L;其浓度2.5 g/L菌丝体干重最高,为1.26 g/100 m L。研究为进一步开展灰树花胞外多糖液体发酵与利用提供了参考。In the present study, Grifola frondosa strain BUAGF- 02 was used for evaluation of glucose andpeptone concentrations on extracellular polysaccharides production. Soluble starch, glucose, yeast extract,peptone, etc were used as basic fermentation medium. 5, 10, 15, 20 and 25 g/L of glucose and 1, 1.5, 2, 2.5 and3 g/L of peptone were used in glucose and peptone assay respectively. The media were shaken and cultured at25℃ and 170 r/min for 10 d followed by a centrifugation. Mycelia and supernatant were collected.Polysaccharides were collected using the ethanol precipitation method. Both mycelia and polysaccharides weredried and weighted. The results showed that yield of polysaccharides reached the highest level of 0.3 g/100 m Lat glucose concentration of 20 g/L, while the highest mycelial weight of 1.82 g/100 m L was achieved at glucoseconcentration of 25 g/L. The yield of polysaccharides reached the highest level of 0.2 g/100 m L at peptoneconcentration of 3 g/L, while the highest mycelial weight of 1.26 g/100 m L was obtained at peptoneconcentration of 2.5 g/L. The study is of certain values to further fermentation researches and applications of G.frondosa polysaccharides.

关 键 词：灰树花 多糖 葡萄糖 蛋白胨 

分 类 号：Q939.9[生物学—微生物学]