桃芽休眠诱导过程中IAA、ABA含量及PpPINs基因表达的变化  

Changes in IAA and ABA Levels and PpPINs Genes Expression During the Bud Dormancy Induction in Peach

作　　者：贾楠[1] 田航[1] 彭竹君[1] 王忆[1] 吴婷[1] 张新忠[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国农学通报》2015年第25期100-106,共7页Chinese Agricultural Science Bulletin

基　　金：国家支撑课题"设施果树和花卉优质高效低耗生产关键技术研究与示范"(2011BAD12B02)

摘　　要：为认识桃芽休眠诱导过程中生理与相关基因表达的变化,分别用HPLC检测‘中农红久保’、‘中农3号’、03-30-035 3个桃品种(系)休眠诱导时期叶片和叶芽IAA和ABA含量,用RT-q PCR检测叶片和叶芽生长素极性运输载体基因Pp PIN1、Pp PIN3、Pp PIN5和Pp PIN8共4个基因的相对表达量。结果表明,在桃芽休眠诱导过程中,叶片IAA含量未见显著变化,ABA含量自10月6日开始升高,至10月16日含量达到高峰;叶芽中10月上中旬IAA含量骤增,而ABA含量逐渐降低。本研究中桃基因组的4个Pp PINs基因家族成员中,叶片中活跃表达为Pp PIN5,其次为Pp PIN3,在10月上中旬Pp PIN5出现强烈表达;而在10月26日,叶芽中活跃表达的Pp PIN1和Pp PIN3表达量骤降。桃休眠诱导过程中叶片和叶芽内内源激素和Pp PINs基因表达量变化明显不同。This study focused on the changes of physiology and relative gene expression during the buddormancy induction in peach. The contents of IAA and ABA were measured by HPLC and the relative genesexpression of auxin polar transport carrier genes Pp PIN1, Pp PIN3, Pp PIN5 and Pp PIN8 was detected by RT-q PCR in leaves and vegetative buds. Three peach varieties,‘Zhongnonghongjiubao＇,‘Zhongnongsanhao＇, 03-30-035, were chosen as the test materials. Results showed that during the bud dormancy induction in peach,the content of IAA did not show a significant change, but the content of ABA rose since October 6 and thenreached the peak on October 16. Nevertheless, the content of IAA surged in leaf buds while the content of ABAdecreased gradually. Among the four genes mentioned above, Pp PIN5, followed by Pp PIN3, was in activeexpression in leaves and had a strong expression in the early to mid-October. However, the relative expressionof Pp PIN1 and Pp PIN3 dropped suddenly in October 26 in vegetative buds. In general, the changes ofendogenous hormones and Pp PINs genes relative expression were significantly different in leaves andvegetative buds during the bud dormancy induction in peach.

关 键 词：桃 芽诱导休眠 激素 PpPINs基因 

分 类 号：S662.1[农业科学—果树学]