丹参多酚酸对持续性正加速度暴露下大鼠牙龈组织的保护作用  

Protective effects of salvia miltiorrhiza polyphenols acid on gingival tissues of the rats under sustained high ＋Gz exposure

作　　者：尹音[1] 李冬霞[1] 冯岩[1] 陈新[1] 王喆[2] 

机构地区：[1]空军总医院口腔科,北京100142 [2]空军总医院临床航空医学实验室,北京100142

出　　处：《中华航空航天医学杂志》2015年第2期130-134,F0002,共6页Chinese Journal of Aerospace Medicine

基　　金：基金项目：全军“十一五”医药卫生科研课题（06MA022）;全军医学科技“十二五”科研课题（BWS12J078）

摘　　要：目的 探讨持续性正加速度（＋Gz）暴露对大鼠牙龈组织的影响以及丹参多酚酸的保护作用. 方法 采用随机区组法将27只雄性SD大鼠分为阴性对照组、＋5Gz阳性对照组、＋5Gz药物组（低、中、高剂量）、＋10 Gz阳性对照组、＋10 Gz药物组（低、中、高剂量）,共9组,每组3只.除阴性对照组外,其余各组动物分别在＋5Gz和＋10 Gz的条件下各离心30 s,每天重复5次,间隔以＋1Gz持续60 s,每周4d,共3周.实验期间给药组于每次离心前30 min按剂量腹腔注射丹参多酚酸溶液（低、中、高给药剂量分别为2、6、18 mg/kg）,3个对照组均注射等体积生理盐水.观察大鼠行为活动的变化.光镜观察各组大鼠牙龈组织形态学变化,并采用PCR法测定牙龈组织中热休克蛋白70（heat shock protein 70,HSP70） mRNA相对含量. 结果 持续性正加速度对大鼠的行为活动有一定程度的影响,采用丹参多酚酸干预后,大鼠的活动程度有逐渐恢复的趋势.各组大鼠牙龈组织呈红色,有光泽,质地坚韧,未见明显肿胀和出血.对照组牙龈组织上皮基本完整;＋5 Gz阳性对照组和药物组的牙龈组织上皮过度角化,粒层明显;＋10 Gz阳性对照组和药物组牙龈组织上皮角化程度更高,粒层更明显.随着正加速度G值的增加,大鼠牙龈组织HSP70 mRNA相对表达量亦增加（F=169.22、650.69,P＜0.01）,随着丹参多酚酸给药浓度的增加,HSP70 mRNA的相对表达量受到明显抑制（F=68.73,P＜0.01）. 结论 HSP70 mRNA相对表达量与＋Gz值的增加呈正相关,＋Gz值越大对大鼠牙龈组织的损伤也越大;丹参多酚酸对＋Gz暴露下的牙龈组织损伤具有一定的保护作用.Objective To investigate changes in gingival tissues of the rats in sustained ＋ Gz acceleration exposure and the protective effects of salvia miltiorrhiza polyphenols acid.Methods Twenty-seven male SD rats were randomly divided into negative control,＋ 5 Gz positive control,＋5 Gz dose group （low,moderate,and high dose）,＋10 Gz positive control and ＋10 Gz dose group （low,moderate,and high dose） groups.Except negative control group,other groups were exposed to ＋5 Gz or ＋10 Gz for 30 s with 60 s intervals,5 times a day,4 days a week for 3 consecutive weeks.Salvia miltiorrhiza polyphenols acid was injected into rat＆#39;s abdomen with the different doses （2,6,18 mg/kg） 30 min before each exposure.Three control groups were injected equivalent amount of 0.9% sodium chloride solution.The rat＆#39;s behavior and gingival tissues changes were observed after the experiments.Real time PCR was applied to determine the relative expression of heat shock protein 70 （HSP70） mRNA in gingival tissues.Results Sustained high ＋Gz exposure influenced the active degree of model rats in a certain range and salvia miltiorrhiza polyphenols acid helped the rats turning to the activity recovery.The gingival tissues of every groups showed red,sheen and with firm and tenacious character,and no obvious plumping and bleeding were observed.The epithelium of gingival tissues of negative control group kept its integrity,but the epithelium of ＋5 Gz positive control and dose groups showed significant keratinization and granular layer.Situations were more serious for ＋10 Gz positive and ＋10 Gz dose groups.The relative expression of HSP70 mRNA was increased with the growing of ＋Gz level （F=169.22,650.69,P〈0.01）,but showed significant restraint with the augment of salvia miltiorrhiza polyphenols acid dose （F=68.73,P〈0.01）.Conclusions The relative expression of HSP70 mRNA in gingival tissues of rats is positively correlated to ＋Gz level.The higher ＋Gz level the more harmful to the gingival tissues

关 键 词：加速度 大鼠 牙龈 HSP70热休克蛋白质类 丹参多酚酸 

分 类 号：R85[医药卫生—航空、航天与航海医学]