星型胶质细胞与小胶质细胞在家兔减压病脊髓损伤中的作用  

Effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness

作　　者：王翠翠[1] 刘晓红[1] 曹永成[1] 毕利泉[1] 耿明[1] 

机构地区：[1]济南军区总医院病理科,济南250031

出　　处：《中华航空航天医学杂志》2015年第2期135-139,F0003,F0004,共7页Chinese Journal of Aerospace Medicine

基　　金：基金项目：全军医学科研“十二五”面上项目（CWS11J241）

摘　　要：目的 探讨星型胶质细胞与小胶质细胞的活化在家兔减压病脊髓损伤中的作用. 方法 成年健康雄性新西兰家兔21只,按随机数字表法分为对照组、安全减压组和减压病（decompression sickness,DCS）组,每组7只.实验动物置于加压舱内.安全减压组参考我国海军空气潜水减压表减至常压;DCS组5 min匀速加压至0.8 MPa（绝对压）,停留60 min后,再5 min匀速减至常压.光镜观察胸腰段脊髓的病理形态学改变;荧光实时定量法测定肿瘤坏死因子-a（tumor necrosis factor-α,TNF-α）、胶质纤维酸性蛋白（glial fibrillary acidic protein,GFAP）基因mRNA表达;免疫组织化学方法测定TNF-α、GFAP和钙结合蛋白（ionized calcium binding adapter molecule 1,IBA1）的表达.结果 DCS组脊髓白质内空泡形成,脊髓中TNF-α基因mRNA相对表达量（6.28＆#177;1.73）与对照组（1.00＆#177;o.14）和安全减压组（1.34＆#177;0.42）相比,差异均有统计学意义（P＜0.01）;GFAP基因mRNA相对表达量（7.39＆#177;2.04）与对照组（1.02＆#177;0.26）和安全减压组（1.63＆#177;0.90）相比,差异均有统计学意义（P＜0.01）.TNF-α蛋白表达量（24.14＆#177;2.61）较对照组（6.71＆#177;1.25）和安全减压组（8.28＆#177;1.11）明显增加,差异均有统计学意义（P＜0.01）;GFAP蛋白表达量（18.20＆#177;4.38）较对照组（4.30＆#177;2.70）和安全减压组（6.20＆#177;2.92）明显增加,差异均有统计学意义（P＜0.01）;IBA1蛋白表达量（21.53＆#177;1.37）较对照组（5.94＆#177;0.36）和安全减压组（6.69＆#177;0.81）明显增加,差异均有统计学意义（P＜0.01）. 结论 星形胶质细胞和小胶质细胞在减压病脊髓损伤的病理生理过程中发挥重要作用,在DCS的早期即可被激活,释放大量的TNF-α,引起过度炎症反应,导致脊髓损伤.Objective To investigate the effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness （DCS）.Methods Twenty-one healthy adult male New Zealand rabbits were averagely divided into 3 groups according to random number table：control group,safety relief group and DCS group.Experimental animals were placed in the pressure cabin.Safety relief group model referred to the Chinese Navy diving decompression tables when decompression applied.In DCS group,the pressure equably was increased to 0.8 MPa （absolute pressure） within 5 min by compressed air,maintained for 60 min and then equably decompressed to normal pressure within 5 min.The change of pathology morphology in the spinal cord of thoracolumbar vertebra was observed by light microscope.The expression of tumor necrosis factor-a （TNF-a） and Glial Fibrillary Acidic Protein （GFAP） mRNA were measured by real-time polymerase chain reaction （real-time PCR） and the expression of TNF-α,GFAP,ionized calcium binding adapter molecule 1 （IBA1） protein was measured by immunohistochemistry method.Results There were some cavity formations on white matter of spinal cord in DCS group.The relative expression of TNF-α mRNA was higher in DCS （6.28＆#177;1.73） than that in control group （1.00＆#177;0.14） and in safety relief group （1.34＆#177;0.42） （P〈0.01）.The relative expression of GFAP mRNA was higher in DCS （7.39＆#177;2.04） than in that control group （1.02＆#177;0.26） and in safety relief group （1.63＆#177;0.90） （P〈 0.01）.The expression of TNF-α protein was significantly higher in DCS group （24.14＆#177; 2.61） than that in control group （6.71＆#177;1.25） and in safety relief group （8.28 ＆#177; 1.11） （P〈0.01）.The expression of GFAP protein was also significantly higher in DCS （18.20＆#177;4.38） than that in control group （4.30＆#177;2.70） and in safety relief group （6.20＆#177;2.92） （P〈0.01）.The expression of IBA1 protein was also sig

关 键 词：减压病 脊髓损伤 星形细胞 小神经胶质细胞 肿瘤坏死因子 

分 类 号：R852.16[医药卫生—航空、航天与航海医学]