雪莲培养细胞提取物的抗急性低压缺氧作用机制研究  

Mechanism research for the resistant effects of Saussurea involucrate cultured cell extracts on acute hypobaric hypoxia

作　　者：周湘洁 邸敏 张丽芬 杜学礼 赵华丽 钟悦 尚颖 周晓春 郭志刚[2] 

机构地区：[1]解放军总装备部后勤部航天城门诊部,北京100094 [2]清华大学化学工程系,北京100084

出　　处：《中华航空航天医学杂志》2015年第1期8-13,共6页Chinese Journal of Aerospace Medicine

摘　　要：目的 探讨雪莲培养细胞提取物的抗急性低压缺氧作用机制. 方法 Wistar大鼠36只,采用数字随机表法平均分为空白对照组、阴性对照组和雪莲培养细胞提取物组.各组动物于每日清晨灌胃,连续14d.其中雪莲培养细胞提取物组给予雪莲培养细胞提取物500 mg/kg灌胃,空白对照组和阴性对照组给予等量蒸馏水.14d后,除空白对照组外,其余两组大鼠行8h的模拟海拔8 000m的急性低压缺氧暴露.断头处死各组大鼠,取血、分离脑组织,检测血清和脑组织中的谷氨酸（glutamic acid,GLU）、NO、乳酸（lactic acid,LD）、乳酸脱氢酶（lactate dehydrogenase,LDH）和丙二醛（malondialdehyde,MDA）的含量,以及总抗氧化能力（total antioxidant capacity,T-AOC）、SOD、ATP酶和谷胱甘肽过氧化物酶（glutathion peroxidase,GSH-PX）的活力. 结果 与空白对照组比较,阴性对照组大鼠脑组织或血清中的GLU、NO、LD、LDH和MDA的含量均显著增加（P＜0.01）;Na＋-K ＋-ATP酶、Ca2＋-Mg2＋-ATP酶、T-AOC、SOD和GSH-PX的活力均显著降低（P＜0.01）.与阴性对照组比较,雪莲培养细胞提取物组大鼠脑组织和血清中的GLU、NO、LD、LDH和MDA的含量均显著降低（P＜0.01）;Na＋-K＋-ATP酶、Ca2＋-Mg2＋-ATP酶、T-AOC、SOD和GSH-PX的活力均显著升高（P＜0.01）. 结论 在模拟海拔8 000 m急性低压缺氧环境下,雪莲培养细胞提取物能有效地清除或抑制氧自由基的生成,预防脑组织和血清中GLU和LD的堆积;通过保护细胞膜上Na＋-K＋-ATP酶和Ca2＋-Mg2＋-ATP酶的功能,预防能量代谢障碍所引起的继发毒性反应,减轻细胞水肿,保护脑组织和细胞的完整性及其功能,有效地阻止急性低压缺氧的损伤.Objective To investigate the resistance to acute hypobaric hypoxia of Saussurea involucrata cultured cell extracts and the underlying mechanism.Methods Thirty-six Wistar rats were averagely divided into blank control group,negative control group and Saussurea involucrate cultured cell extracts group according to random number table.The Saussurea involucrate cultured cell extracts were infused to the rats of Saussurea involucrate cultured cell extracts group with the dose of 500 mg/kg for 14 d while the distilled water was fed to other 2 groups in the period.Except blank control group,the rats of other groups were exposed in simulative 8 000 m altitude experiencing acute hypobaric hypoxia after 14 d infusion.Then the rats of all groups were killed and sampled blood serum and brain tissue.The changes of various biochemical factors in brain tissue and blood serum,such as glutamic acid （GLU）,lactic acid （LD）,lactate dehydrogenase （LDH）,malondialdehyde （MDA）,total antioxidant capacity （T-AOC） and glutathion peroxidase （GSH-PX） were tested.Results Comparing with those of blank control group,the GLU,NO,LD,LDH and MDA in blood serum and brain tissue of negative control group were significantly increased （P〈0.01）,and Na＋-K＋-ATPase,Ca2＋ Mg2＋-ATPase,TAOC,SOD and GSH-PX were significantly decreased （P〈0.01）.The GLU,NO,LD,LDH and MDA of Saussurea involucrate cultured cell extracts group were significantly lower than those of negative control group （P〈0.01） but with significantly higher Na＋-K ＋-ATPase,Ca2＋-Mg2 ＋-ATPase,T-AOC,SOD and GSH-PX （P〈0.01）.Conclusions For simulated 8 000 m hypoxia exposure,Saussurea involucrata cultured cell extracts can effectively remove or inhibit the generation of excessive oxygen free radicals and may prevent glutamic acid and lactic acid from accumulating in rat brain tissue and blood serum.By protecting the function of Na-K＋-ATPase and Ca2＋-Mg2＋-ATPase on cell membrane and preventing the consequent toxic reaction induced by e

关 键 词：细胞 培养的 细胞提取物 缺氧 雪莲 

分 类 号：R85[医药卫生—航空、航天与航海医学]