芍药苷对原发性干燥综合征患者外周血单个核细胞分泌细胞因子IL-1、IL-6的影响  

Effects of paeoniflorin on IL-1 and IL-6 production released by peripheral blood mononuclear cell in patients with primary Sj?gren's syndrome

作　　者：于静雅 陈勇 彭勇 李明才[2] 邬秀娣 张振 高巧艳[2] 龚琼瑶 

机构地区：[1]宁波市第二院,宁波315000 [2]宁波大学医学院

出　　处：《现代实用医学》2015年第8期984-986,共3页Modern Practical Medicine

基　　金：浙江省中医药科学研究基金计划(A类)(2013ZA117)

摘　　要：目的从细胞因子分泌调控水平研究芍药苷(PF)抗炎抗风湿的作用机制。方法收集20例原发性干燥综合征(p SS)患者空腹肘静脉血于肝素抗凝管中,用Ficoll-hypague密度梯度离心分离外周血单个核细胞(PBMC),分为PF干预组和不干预组。体外干预培养,分离细胞培养上清,用酶联免疫吸附试验(ELISA)法检测不同浓度PF对三磷酸腺苷(ATP)刺激下该细胞分泌白细胞介素(IL)-1,IL-6的影响。结果 2、20、80 mol/L PF作用4 h,PBMCs培养上清中IL-1、IL-6水平与不干预组比较,差异均无统计学意义(均>0.05);100、150 mol/L PF作用4 h,PBMCs培养上清中IL-1、IL-6水平与不干预组比较,差异均有统计学意义(均<0.05)。结论 PF可能通过下调p SS患者PBMC的IL-1,IL-6分泌发挥对p SS患者的治疗作用,且具有剂量依赖性。Objective To determine the effects ofpaeoniflorin （PF） on production of ATP-induced pro-inflamma- tory cytokines released by peripheral blood mononuclear cells （PBMCs） in patients with primary Sjgren＇s syn- drome （pSS）. Methods Twenty-two pSS patients were included and their fasting venous blood in the heparin tubes were used to isolate peripheral blood mononuclear cells（PBMC） by FicoU-hypague density gradient centrifugation. There were PF intervention group and non-intervention group. With in vitro culture, cell culture supernatant was in- solated and the amounts ofIL-1β and IL-6 in the supernatant produced by these cells were quantitated by enzyme-lin- ked immuno sorbent assay （ELISA）, and then, the regulating effects of PF were evaluated. Results Incubating PBMCs with two, 20, 80μmol/L PF for four hours, there were no statistical significances of IL-1β between PF inter- vention group and non-intervention group[（3 274±90.80）vs（3 405±81.48）, （3 214：i：81,12）vs（3 405a：81.48）, （3 160±97.63）vs（3 405±81.48）, allP 〉 0.05]; Incubating PBMCs with 100, 150 μmol/L PF for four hours, there were statistical significances [（2 819±155.90）vs（3 405±81.48）, （2 383±131.6）vs（3 405±81.48）, all P 〈 0.05]. Incubating PBMCs with two, 20, 80μmol/L PF for four hours, there were no statistical significances of IL-6 between PF interv ention group and non-intervention group [（55 830±1 680）vs（53 110±1 563）, （49 410± 101）vs（53 110±1 101）, （49 120± 1 550）vs（53 110±563）, all P 〉 0.05]; Incubating PBMCs with 100, 150 μmol/L PF for four hours, there were statistical significances [（46 540d：1 149） vs（53 110±1563）, （42 880±1 664）vs（53 110±1 563）, allP 〈 0.05]. Conclusions PF has therapeutic effect on pSS patients by reducing secretion of IL-1β and IL-6 in a dose dependent manner.

关 键 词：芍药苷 原发性干燥综合征 白细胞介素-1 白细胞介素-6 

分 类 号：R593.2[医药卫生—内科学]