Immunohistochemical Study of Hexose Transporters GLUT-2 and GLUT-5 in Birds Gastrointestinal Tract  

作　　者：Piret Hussar Florina Popovska-Percinic Tonu Jarveots Ilmars Duritis Martin Kamer Katerina Blagoevska Lazo Pendovski 

机构地区：[1]Institute of Anatomy, University of Tartu, Tartu 50411, Estonia [2]Faculty of Veterinary Medicine, Ss. Cyril & Methodius University in Skopje, Skopje 1000, Republic of Macedonia [3]Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu 51014, Estonia [4]Faculty of Veterinary Medicine, Latvian University of Agriculture, Jelgava L V-3004, Latvia [5]Faculty of Science and Technology, University of Tartu, Tartu 51014, Estonia

出　　处：《Journal of Agricultural Science and Technology(A)》2015年第3期194-198,共5页农业科学与技术（A）

摘　　要：The hexoses glucose, galactose and fructose serve as important dietary energy sources in animals and glucose plays a central role in energy homeostasis within eucaryotic cells. As relatively little is known about patterns of hexose transporters expression in birds gastrointestinal tract, the aim of the study was to examine glucose transporters 2 （GLUT-2） and glucose transporters 5 （GLUT-5） expression in stomach and duodenal epithelium of two different species--broilers （Gallus gallus domesticus） and ostriches （Struthio camelus var. domesticus） chicken. Materials from the two parts of gastrointestinal tract were collected from six 7-day-old female broilers and six 7-day-old female ostriches. Specimens were fixed with 10% formalin, embedded into paraffin, cut into 7 lain thick slices, followed by immunohistochemical staining with polyclonal primary antibodies rabbit anti-GLUT-2 and rabbit anti-GLUT-5; the staining was carried out according to the manufacturers guidelines （IHC kit, Abcam, UK）. The investigation showed that the staining for both antibodies was more intensive in the epithelial cells of stomach and duodenum of the 7-day-old broilers. In duodenal epithelium, goblet cells and brush border membranes were stained in both species, however the signal was stronger for GLUT-5 than GLUT-2. Staining for GLUT-2 and GLUT-5 occurred in different parts of gastrointestinal tract of 7-day-old ostriches, but was weaker compared to 7-day-old broilers, which showed that the gastrointestinal tract of 7-day-old female broilers was more developed for transportation ofhexoses than 7-day-old female ostriches.

关 键 词：Birds gastrointestinal tract GLUT-2 GLUT-5 hexose. 

分 类 号：S852.2[农业科学—基础兽医学] Q51[农业科学—兽医学]