2012-2014年安康市流感病毒分离结果及分析  

Isolation and analysis of influenza virus in epidemic season Ankang city 2012-2014

作　　者：杜冬冬[1] 刘斌[1] 吴建平[1] 周惠萍[1] 李湘平[1] 刘万静 原凌云[1] 

机构地区：[1]安康市疾病预防控制中心生物检验科,陕西安康725000

出　　处：《中国卫生工程学》2015年第4期363-365,共3页Chinese Journal of Public Health Engineering

摘　　要：目的对安康市2012-2014年流行期流感病毒进行分离鉴定及分析,了解安康市流感流行动态,探索流行规律。方法采集流感样病例(ILI)的咽拭子标本先用real-time PCR检测,阳性标本用狗肾传代细胞(MDCK)进行病毒分离,采用血凝抑制方法(HI)进行流感病毒型别鉴定。结果 2012年10月至2014年3月共监测1所哨点医院ILI咽拭子标本905份,其中PCR阳性171份;分离出流感病毒97株,其中新甲型H1N1 62株,季节性A(H3N2)11株,B型(yamagata)24株。结论 2012-2014年流感流行季节中安康市流行的优势毒株为新甲型H1N1,12月至次年1月为流行高峰,有其他型别的交替流行。Objective To isolate the influenza viruses and analyze their activities in the epidemic seasons from 2012 to 2014 in Ankang city in order to explore epidemic rule. Methods Throat swab specimens collected from the ILI cases were firstly tested with real - time PCR. And the positive specimens were isolated by MDCK cells. Then, the identification of the isolate was carried out with HI （ hemagglutination and hemagglutination inhibition） tests. Results A total of 905 throat swab specimens were collected for detection from October 2012 to March 2014 from one sentinel hospital, including 171 pos- itive PCR specimens. And 97 strains of influenza virus were isolated, in which 62 strains were the pandemic H1N1 virus, 11 strains were A（H3N2） subtype and 24 strains were B - yamagata. Conclusion From 2012 to 2014 in Ankang city, the majority of virus strains in influenza seasons is the pandemic H1 N1 virus, and it reaches the peak from December to next January, and other epidemic viruses are alternating.

关 键 词：流感病毒 MDCK细胞 分离鉴定 

分 类 号：R511.7[医药卫生—内科学]