胰岛素对糖尿病大鼠睾丸脂联素及其受体表达的影响  

Effect of insulin on the expression of adiponectin and its receptors in the testes of type 1 diabetic rats

作　　者：闫旭红 郭志新[1] 吴杰萍[1] 王蕾[1] 景晓锐 刘佳[1] 

机构地区：[1]山西医科大学第二医院内分泌科,太原030001

出　　处：《中华糖尿病杂志》2015年第8期507-512,共6页CHINESE JOURNAL OF DIABETES MELLITUS

基　　金：山西省自然科学基金资助项目（2013011047-1）;山西省留学回国人员科技活动择优资助项目

摘　　要：目的：探讨胰岛素对1型糖尿病大鼠睾丸脂联素、脂联素受体及其介导的信号转导通路表达的影响。方法30只6周龄雄性SD大鼠（体重180-200 g）以随机数字表法分为正常对照组（A组，n=8）和链脲佐菌素注射组（n=22）。链脲佐菌素用于制备1型糖尿病大鼠模型。造模成功的16只雄性SD大鼠随机分为糖尿病组（B组，n=8）和糖尿病胰岛素治疗组（C组，n=8）。C组大鼠每日皮下注射精蛋白锌胰岛素3-5 U，使血糖控制于10 mmol/L左右，A、B组大鼠每日皮下注射等量的生理盐水。于实验8周末留取血标本，用于血生化指标、胰岛素、脂联素及性激素水平检测，然后处死动物，迅速取出双侧睾丸及附睾，计数精子数量及活动率，测定睾丸脂联素及其受体、单磷酸腺苷激活蛋白激酶（AMPK）、蛋白激酶B（AKT）、内皮型一氧化氮合酶（e-NOS）、一氧化氮（NO）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的表达水平。统计采用LSD-t检验和单因素方差分析。结果与A组比较，B组糖尿病大鼠睾丸组织出现显著病理改变，睾丸重量、精子数量及活动率均显著降低（t=3.899、21.139、26.770，均P〈0.05）；睾丸组织表达脂联素及其受体1、p-AMPK/AMPK水平显著降低（A组均为1.00±.00，B组分别为0.66±.09、0.68±.05、0.34±.11，t=8.658、14.297、5.551，均P〈0.05）；p-AKT/AKT、e-NOS蛋白表达水平显著升高（1.54±.27比1.00±.00、1.56±.26比1.00±.00，t=5.083、4.997，均P〈0.05）。胰岛素治疗能显著逆转上述各项指标的变化。与B组相比，C组大鼠睾丸组织病变减轻，睾丸重量、精子数量及活动率降低升高（t=3.444、18.453、23.103,均P〈0.05）；脂联素及其受体1、p-AMPK/AMPK表达水平显著升高（分别为0.85±.11比0.66±.09、0.82±.06比0.68±.05、0.87±.40比0.34±.11，t=4.969、6.151、4.454，均P〈0.05）；p-AKT/AKT、e-NOS蛋白表达水平显著�Objective To explore the effect of insulin on the expression of adiponectin, adiponectin receptors, and adiponectin receptor-mediated signaling pathways in the testes of type 1 diabetic rats. Methods Thirty male 6-week Sprague-Dawley（SD） rats（body weight： 180-200 g） were randomly divided into normal control group （group A, n=8） and streptozotocin injection group（n=22） by using random number table. Type 1 diabetic model was established by intraperitoneal injection of streptozotocin. Sixteen successfully-induced diabetic rats were randomly divided into diabetic group（group B, n=8） and diabetic treated with insulin（group C, n=8）. Rats in group B were given subcutaneous protamine-zinc insulin in does of 3-5 U daily to control blood glucose at 10 mmol/L. The other two groups were given equal volume of normal saline daily. At the end of the eight-week of experiment, rats were sacrificed after blood samples were 〈br〉 collected, and then the bilateral testes were collected quickly. Blood samples were used to detect the levels of biochemical index, insulin, adiponectin and sex hormone. Sperm number and motility were counted. The expression of testicular adiponectin, adiponectin receptors, adenosine 5′-monophosphate （AMP）-activated protein kinase（AMPK）, protein kinase B（AKT）, endothelial nitric oxide synthase（e-NOS）, nitric oxide（NO）, interleukin-6（IL-6）, and tumor necrosis factor alpha（TNF-α） were assayed. Statistical analysis was performed by using LSD-t test and one way ANOVA. Results There were significant pathological changes in the testes of group B than those in group A. Compared with group A, the level of testes weight, sperm number and motility were decreased in group B（t=3.899, 21.139, 26.770, all P〈0.05）, the testicular adiponectin and its receptor 1, the ratio of p-AMPK to AMPK were significantly decreased in group B （（0.66±.09） vs （1.00±.00）, （0.68±.05） vs （1.00±.00）, （0.34±.11） vs （1.00±.00）, t=8.658, 14.297, 5.

关 键 词：糖尿病 1型 脂联素 睾丸 大鼠 胰岛素 

分 类 号：R587.1[医药卫生—内分泌]