测试片法在金黄色葡萄球菌感染快速检测中的应用  

Application of testing slips for rapid detection of Staphylococcus aureus bacterial infections

作　　者：韩善桥[1] 赵晓航[1] 石峰[1] 史成和[1] 

机构地区：[1]中国人民解放军海军总医院基础医学研究中心,北京100048

出　　处：《国际检验医学杂志》2015年第17期2459-2460,2463,共3页International Journal of Laboratory Medicine

基　　金：全军医药卫生科研基金课题(CHJ12J025;BWS13J012);国家高技术研究发展计划("863"计划)资助项目(2013AA041201)

摘　　要：目的探讨金黄色葡萄球菌测试片在检测临床细菌感染中的应用。方法以金黄色葡萄球菌、大肠埃希菌、阴沟肠杆菌和肺炎克雷伯菌等26种标准菌株测定测试片的灵敏度和特异性,对测试片检测细菌的最佳培养时间及储存稳定性进行实验。分离培养法细菌鉴定使用全自动微生物分析仪。对测试片法和分离培养法测定临床标本的结果进行分析。结果金黄色葡萄球菌测试片对纯菌检测灵敏度为1CFU/mL,与24种非目的菌无交叉反应,24h完成测定,25℃条件存放2周无明显影响,使用测试片法检测临床标本结果与分离培养法一致。结论金黄色葡萄球菌测试片法灵敏度高、特异性强、简单快速,在特殊条件下可用于检测临床标本中的金黄色葡萄球菌。Objective To investigate the application of testing slip method for the rapid detection of Staphylococcus aureus bac‐terial infections .Methods The specificity and sensitivity of a quick testing slip for Staphylococcus aureus were determined by using 26 kinds of standard reference bacterial strains ,including Staphylococcus aureus ,Escherichia coli ,Enterobacter cloacae and Klebsiel‐la pneumoniae .Incubation time and storage stability of the testing slips were tested .Isolation and identification of bacteria were per‐formed by using VITEK auto microbe system .Results The detection limit of the quick testing slip was 1 CFU/mL for pure bacte‐rial culture ,and the slip had no cross‐reaction with 24 non‐target strains .Twenty‐four hours were required to finish the entire test . There was no significant change when the testing slip was stored at 25 ℃ for 2 weeks .The quick testing slips were used to detect Staphylococcus aureus in clinical specimens ,and the results were consistent with those of the traditional method .Conclusion The quick testing slips were highly sensitive ,specific ,simple and can be used to detect Staphylococcus aureus quickly in clinical speci‐mens .

关 键 词：金黄色葡萄球菌 快速测试片 细菌 感染 

分 类 号：R446.5[医药卫生—诊断学]