赖氨大黄酸减轻百草枯中毒小鼠心脏损伤  

Rhein lysinate attenuated paraquat-induced cardiac injury in mice

作　　者：李开济 郝晓方 章广玲 魏洁[2] 林雅军[2] 魏静波 甄永占 

机构地区：[1]华北理工大学基础医学院河北省慢性疾病重点实验室唐山市慢性病临床基础研究重点实验室,河北唐山063000 [2]卫生部北京医院/北京老年医学研究所,北京100730

出　　处：《基础医学与临床》2015年第9期1171-1175,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81001439);河北联合大学大学生创新性实验计划(X2014078)

摘　　要：目的观察赖氨大黄酸(RHL)对百草枯中毒小鼠心脏损伤的保护作用及其机制。方法小鼠随机分为对照组、百草枯模型组(腹腔注射百草枯建立活性氧自由基引起心脏损伤的小鼠模型)及RHL治疗组(造模前1周给予RHL(50 mg/kg)。用硫代巴比妥酸法检测心脏组织MDA含量,用联苯三酚自氧化法检测SOD活性,用NADPH偶联法检测GSH-Px活性。HE染色及DCFH-DA染色分别观察心脏病理改变及活性氧水平;Western blot检测心肌衰老相关基因的表达。结果与对照组相比,百草枯模型组小鼠心脏组织SOD和GSH-Px活性下降(P<0.05),MDA水平升高(P<0.05),RHL能减轻百草枯的作用(P<0.05);百草枯引起心肌结构破坏,心肌组织活性氧水平升高,RHL减轻上述变化(P<0.05)。百草枯能够降低SIRT1的表达,增加P53的乙酰化及P53和P66的表达。RHL减轻上述变化(P<0.05)。结论 RHL可以减轻百草枯中毒后所致的小鼠心脏损伤。Objective To investigate the protective effects of rhein lysinate （RHL） on cardiac tissue damage in- duced by paraquat in experimental mice, and to clarify its mechanism. Methods In this study mice were assigned to the following three groups： control, paraquat model, and RHL-treated groups. The model of oxidative damage mice was established by intraperitoneal injection of paraquat. RHL-treated group was given RHL （50 mg./kg） by gavage for one week before performing model. The other two groups were given equal volume of distilled water. For making model, paraquat was intraperitoneally injected in the paraquat model and RHL-treated group. The content of MDA was detected by thiobarbiturie acid assay. The activities of SOD and GSH-Px were detected by biphenyl three phenolic autoxidation assay and NADPH coupling method respectivly. The pathological profile of cardiac tis- sue was observed by hematoxylin and eosin （HE） staining and staining. The change of proteins related to myocardial damage control group, the activities of SOD and GSH-Px decreased （P 〈 reactive oxygen species was observed by DCFH-DA detected by Western blot. Results Compared with 0.05 ） and the content of MDA increased（P 〈 0. 05 ） in paraquat model group. However, these changes were attenuated byr RHL treatmen （P 〈 0. 05 ）. The pathologi- cal examination indicated the structure of cardiac tissue was damaged and reactive oxygen species of cardiac tissue was increased after paraquat was given, however, these changes were attenuated after RHL treatmen. It was shown in western blot analysis that compared with control group, the expression of SIRT1 decreased, the acetylation of P53 and the expression of P53 and P66 increased in paraquat-treated group. These changes were attenuated by RHL treatmen （P 〈 0. 05）. Conclusions RHL may attenuate paraquat-induced cardiac injury in mice.

关 键 词：赖氨大黄酸 百草枯 心脏损伤 活性氧自由基 

分 类 号：R595.4[医药卫生—内科学]