两株极地真菌次级代谢产物的研究  

RESEARCHES OF THE SECONDARY METABOLITES OF TWO ARCTIC-DERIVED FUNGUS

作　　者：闫凝星 方莎莎[1] 刘靖堂 卢小玲[1] 刘小宇[1] 焦炳华[1] 

机构地区：[1]第二军医大学生物化学与分子生物学教研室,上海200433

出　　处：《极地研究》2015年第3期319-325,共7页Chinese Journal of Polar Research

基　　金：国家高技术研究发展计划(863计划)(2012AA092105);广东省海洋局公益项目(GD2012-D01-001);国家自然科学基金(41306197)资助

摘　　要：采用硅胶柱层析、凝胶柱层析和HPLC等色谱技术,对极地真菌Articulospora sp.Z1-1和Penicilliumsp.S-1-10的发酵液提取物进行分离纯化,共得到9个化合物,通过理化性质及波谱学分析并与相关文献比对,确定化合物结构为3,7-二羟基-9-甲氧基-1-甲基-苯并[c]色烯-6-酮(1),1,4-二羟基甲苯(2),间羟基苄醇(3),对羟基苯乙醇(4),邻苯二甲酸二丁酯(5),1,7-二羟基-3-羟甲基-8-甲氧基-二苯并[b,e]氧杂卓-13,14-二酮(6),邻苯二甲酸二(2-乙基己)酯(7),4-十三烷基-苯甲醛(8)和(Z)-7-十四烯酸(9)。其中,化合物1—6首次从Articulospora sp.属真菌中获得,化合物8为新天然产物。化合物1和4被证明具有抑制稻瘟霉的作用,化合物2有较好的抗菌和细胞毒活性,化合物3有较强的抗氧化活性。Nine compounds were obtained fromthe fermentation broth of the Arctic-derived fungus Articulospora sp. ZI-1 and Penicillium sp. S-1-10. The broth extract was isolated and purified by column chromatography on silica-gel, Sephadex LH-20 and HPLC. The structures were elucidated by physical and chemical properties and spectral analysis, and comparison with the data of literatures. The nine compounds were identified as 3,7-dihydroxy-9-methoxy- 1-methyl-benzo [ c ] chromen-6-one （ 1 ）, 1,4-dihydroxytoluene （ 2 ）, 3-hydroxymethyl phenol （ 3 ）, 2-hydroxy- phenethylalcohol （4）, dibutyl phthalate （ 5 ）, 1,7-dihydroxy-3-hydroxyl-8-methoxy-dibenzo [ b, e ] oxepine-13, 14- dion-e （ 6）, bis （2-ethylhexyl） phthalate （7）, 4-tridecyl-benzaldehyde （ 8 ） and （Z） -7-tetradecenoic acid （ 9 ）. Compounds 1-6 were firstly isolated from Articulospora sp. Z1-1 genus and compound 8 was new natural product. Compounds 1 and 4 exhibited antifungal activityagainst conidia of Pyricularla oryzae, and compound 2 was shown to have antibacterial and cytotoxic activities, and compound 3 exhibited strong antioxidant activity.

关 键 词：极地真菌 节枝孢属 青霉属次级代谢产物 生物学活性 

分 类 号：Q936[生物学—微生物学]