钴60-γ射线辐照对动物源性膜材指示病毒灭活效果的验证  

Verification of inactivation efficacy of indicator viruses in animal origin membrane by Co 60-γ ray irradiation

作　　者：张伟 潘洁丽 钱垂文[2] 徐斌 王一飞[2] 

机构地区：[1]广东冠昊生物科技股份有限公司再生型医用植入器械国家工程实验室,广东广州510530 [2]暨南大学生物医药研发基地基因工程药物国家工程研究中心,广东广州510632

出　　处：《中国卫生检验杂志》2015年第16期2666-2668,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省重大科技专项(2010A080407005);广东省第一批战略性新兴产业核心技术攻关项目(2011A08-1402001)

摘　　要：目的验证钴60-γ射线辐照对动物源性膜材中模拟感染指示病毒的灭活效果。方法将3个连续批次的动物源性膜材中间品,按1∶10(m/V)加入预先测定滴度的CVB3、PRV、BVDV和PPV病毒。设立未经辐照的病毒为对照组,试验组经25 k Gy钴60-γ射线辐照后,提取浸提液,采用96孔板细胞病变(CPE)法观察各梯度稀释液的细胞病变情况,按照Reed-Muench法计算对照组和试验组中4种指示病毒的lg TCID50/0.1 ml,并对比辐照前后病毒滴度的变化情况。结果经25 k Gy钴60-γ射线辐照预先感染病毒的动物源性膜材后,实验结果显示4种指示病毒均不能使宿主细胞出现病变,各病毒滴度对数值变化均>4 logs,符合国家相关法规对动物源性材料病毒灭活效果的要求。结论25 k Gy钴60-γ射线辐照可以作为灭活动物源性膜材中潜在病毒的有效方法。Objective To verify the inactivation efficacy of indicator viruses in animal origin membrane by Co 60- γ ray irradiation. Methods Adding a pre- determined titers of CVB3,PRV,BVDV and PPV viruses by 1∶10（ m / V） in three consecutive batches of animal origin membranes intermediate products. The unirradiated viruses was used as the control group,the experimental group after the irradiation with 25 k Gy Co 60- γ ray,extracted leaching liquor,then 96 well plate cell lesions（ CPE）were used to observe the pathological changes of the cells of the gradient dilution. Then we calculated the lg TCID50/0. 1 ml of four indicator viruses in control group and experimental group by the Reed- Muench method,and compared changes in viral titer before and after irradiation. Results Four indicator viruses in animal origin membrane can＇t make the host cells lesion after25 k Gy Co 60- γ ray irradiation,and all logarithmic changes in viral titer are greater than 4 logs,which is consistent with the requirements of the relevant national laws and regulations about animal origin materials virus inactivation. Conclusion The Co 60- γ ray irradiation at a dosage of 25 k Gy can be used as an effective method to inactivate potential viruses of animal origin membrane.

关 键 词：Γ射线 动物源性膜材 指示病毒 病毒灭活 

分 类 号：R187[医药卫生—流行病学]