利用VIGS技术对抗SMV候选基因GmZ15的功能分析  

Analysis on the Candidate Resistance Gene GmZ15 to Soybean Mosaic Virus by VIGS

作　　者：黄赛花[1] 郑桂杰[1] 杨永庆[1] 智海剑[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《大豆科学》2015年第4期582-587,共6页Soybean Science

基　　金：国家自然科学基金(31171574;31371646);国家现代农业产业技术体系(CARS-004);转基因生物新品种培育专项(2008ZX08004-004)

摘　　要：大豆花叶病毒(SMV)是我国大豆田间主要病毒性病害,阐明大豆对SMV的抗性机制对抗性的利用具有重要意义。利用VIGS技术对抗性基因RSC3Q的候选基因Gm Z15进行功能分析,结果表明:Gm Z15在SMV的诱导后表达量发生明显变化,在接种后24 h表达量达到最高。沉默Gm Z15后的表达量分别为缓冲液对照和p GG7R2空载体对照组的0.3和0.2倍,差异极显著,表明Gm Z15被有效沉默。沉默后接种SMV结果显示,沉默后48 h SMV的浓度约是未沉默处理的6倍,说明沉默Gm Z15有助于SMV的积累,但在其上位叶中均检测到少量SMV且差异未达到显著水平,由此推测Gm Z15介导着一种大豆对SMV的防御反应,齐黄1号对SC3株系的抗性另有其它基因参与。Soybean mosaic virus（ SMV） is one of the most prevalent pathogens in soybean production in China. To clarify the mechanism for SMV resistance would be great significant. VIGS（ virus induced gene silence） was used to analyze the functions of candidate gene Gm Z15 for resistance locus RSC3 Q. The results showed the expression of Gm Z15 changed greatly after inoculated with SMV,and reached a peak 24 hours later. After Gm Z15 was silenced,the expression of Gm Z15 dropped to 0. 3compared with the control inoculated with buffer and 0. 2 compared with the control inoculated with empty vector p GG7R2.The silencing of Gm Z15 contributes to the accumulation of SMV in soybean. The accumulation of SMV was 6 times more in soybean which Gm Z15 was silenced than in the control. While,there was no significant difference in the upper leaf. So we speculated that Gm Z15 did induce a kind of defense reaction. There may be other gene（ s） involved in the resistance process in Qihuang 1.

关 键 词：大豆花叶病毒 病毒诱导的基因沉默 抗病基因 功能验证 

分 类 号：S565.1[农业科学—作物学]