不同磷浓度下结瘤对大豆生长及苹果酸合成和分泌的影响  

Effects of Nodulation on Soybean Growth,Malate Synthesis and Exudation in Different Phosphorus Concentrations

作　　者：李姣姣[1] 徐舟[1] 梁翠月[1] 廖红[1] 

机构地区：[1]华南农业大学农学院/亚热带农业生物资源保护与利用国家重点实验室/根系生物学研究中心,广东广州510642

出　　处：《大豆科学》2015年第4期643-647,652,共6页Soybean Science

基　　金：国家重点基础研究发展计划"973计划"(2011CB100301);国家自然科学基金(31301835)

摘　　要：以大豆品种粤春03-3为研究对象,采用水培的方式,在高低磷条件下,分别进行接种或不接种根瘤菌的处理。在大豆花期和成熟期分别测定大豆干重、氮磷浓度、根系苹果酸浓度和分泌速率,以研究结瘤对磷调控大豆生长及苹果酸合成和分泌的影响。结果表明:在低磷条件下,结瘤对大豆植株的干重、氮磷浓度均没有显著影响;在高磷条件下,结瘤能够显著提高植株地上部干重和氮浓度。在不结瘤条件下,磷对根系苹果酸浓度没有显著影响。但结瘤后,低磷明显增加了成熟期根系苹果酸的浓度。而且,结瘤显著提高了高磷条件下根系苹果酸的分泌速率。这些结果表明,结瘤影响了磷对大豆生长、根系苹果酸合成和分泌的调控。The objective of study was to evaluate the effects of nodulation on the response of malate synthesis and exudation from soybean roots to phosphorus（ P） availability. One soybean genotype YC03-3 was used,and inoculated with or without rhizobia under both high and low P conditions. At both flowering and maturing stages,plant dry weight,nitrogen（ N） and P concentration,malate concentration and exudation were measured. The results showed that nodulated plants had higher biomass and N / P concentration than those in non-nodualted plants under high P conditions. However,no significant difference was observed under low P conditions. Malate concentration in non-nodulated roots was not influenced by P availability. However,malate concentration in nodulated roots was significantly higher at low P level than that at high P level at maturing stage.Moreover,malate exudation was significantly higher in the nodulated plants than that in non-nodulated plants under high P conditions. The results indicated that nodulation was involved in regulation of phosphate starvation responsive soybean growth,malate concentration and exudation.

关 键 词：磷 根瘤菌 大豆 苹果酸 

分 类 号：S565.1[农业科学—作物学]