真菌发酵滤液对大豆胞囊线虫二龄幼虫趋性的影响  

Effects of Parasitic Fungal Fermentation Filtrate on Chemotaxis of Soybean Cyst Nematode Juvenile

作　　者：鲁建聪 宋洁[1,2] 许艳丽[2] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]中国科学院东北地理与农业生态研究所/黑土区农业生态院重点实验室,黑龙江哈尔滨150081

出　　处：《大豆科学》2015年第4期671-674,678,共5页Soybean Science

基　　金：国家自然科学基金(39071900)

摘　　要：采用滤纸片法和直接法,通过考察大豆胞囊线虫二龄幼虫（J2）在WA平板上不同区域内的分布率,研究淡紫拟青霉（Paecilomyces lilacinus）、镰孢菌属（Fusarium spp.）和厚垣轮枝菌（Pochonia chlamydosporium）的12个不同菌株代谢物滤液对大豆胞囊线虫J2趋性的影响。滤纸片法结果显示,WA培养基上0~1 cm区间内,大豆胞囊线虫二龄幼虫（J2）在12个寄生真菌菌株代谢物滤液中分布率均较低,F-9-3、V-2、F-5和V-1菌株代谢物滤液中的分布率分别为17.3%、17.3%、18.0%和18.3%,显著低于对照水的分布率26.0%,也低于J2在根系分泌物中的分布率32.3%（P〈0.05）。直接法结果与滤纸片法基本一致,大豆幼根蘸取寄生真菌代谢物滤液后,线虫幼虫在0~1 cm区间内分布率范围为18.0%~31.3%,显著低于幼根蘸清水对照（P〈0.05）,说明大豆胞囊线虫J2对12个寄生真菌菌株代谢物滤液存在一定的趋避性。The soybean cyst nematode（ SCN） is one of the serious diseases in soybean production. With the current emphasis on environmental pollution and the demand for pollution-free agricultural products,nematodes biological control research received more attention. The effect of 12 parasitic fungal isolates filtrates which were made by Paecilomyces lilacinus,Fusarium spp. !and Pochonia chlamydosporium was tested on SCN. It was investigated by investigated the difference of J2 distribution rate on WA. The methods of filtering paper and direct test were conducted in our laboratory. Both methods showed that SCN J2 were repelled by 12 parasitic fungal isolates filtrates in different degrees. The results of filtering paper method showed that the distribution rate of J2 with treatments of 12 parasitic fungal isolates filtrates was low on 0-1 cm area. The distribution rates of soybean cyst nematode juvenile（ J2） with the treatment of F-9-3,V-2,F-5 and V-1 isolates filtrates were 17. 3%,17. 3%,18. 0% and 18. 3% in the 0-1 cm area,respectively. It was significantly lower than control in water and root diffusate by26. 0% and 32. 3%（ P〈0. 05）. The same result was indicated in direct test. The distribution rates of soybean cyst nematode Juvenile（ J2） were between 18. 0%-31. 3%,in 0-1 cm area with the treatments of soybean root dipped with 12 parasitic fungal fermentation filtrates. It was significantly lower than the control. It was proved that soybean cyst nematode juveniles（ J2） were repelled by 12 parasitic fungal fermentation filtrates.

关 键 词：大豆胞囊线虫二龄幼虫 寄生真菌 趋性 发酵滤液 根浸提液 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]