漫反射光谱测量皮窗模型中血管的血氧饱和度  

Measurement of Oxygen Saturation for Blood Vessels in Dorsal Window Chamber Using Diffuse Reflection Spectroscopy

作　　者：李艺容 林黎升[1] 刘丽娜[1] 陈德福[1,2] 顾瑛[2] 李步洪[1] 

机构地区：[1]医学光电信息科学与技术教育部重点实验室福建省光子技术重点实验室,福建福州350007 [2]中国人民解放军总医院激光医学科,北京100853

出　　处：《激光生物学报》2015年第4期320-325,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(61275216);国家卫计委科学研究基金(WKJ-FJ-30);福建省自然科学基金项目(2014J07008;2014J01225)

摘　　要：血管中的血氧饱和度(Oxygen saturation,St O2)作为影响血管靶向光动力疗法(Vascular targeted photodynamic therapy,V-PDT)疗效的关键要素之一,实验测量了活体裸鼠背皮窗模型中血管的漫反射光谱(450-800nm),并通过拟合漫反射光谱数据定量获得了血管中的St O2。同时,研究了高氧、低氧和常氧等三种不同氧条件下V-PDT中血管的St O2和血管管径的变化情况。结果表明,高氧和常氧条件下的平均St O2和血管收缩较为显著,但低氧组的平均St O2和血管收缩不明显。在相同氧条件和不同光照功率密度条件下,V-PDT前后靶向血管的平均St O2与血管管径的变化之间没有显著相关性,但V-PDT前后平均St O2的变化量与光照功率密度之间呈正相关。Monitoring oxygen saturation changes during vasculartargeted photodynamic therapy（ V-PDT） is crucial for achieving an optimal therapeutic outcome. In this study,the diffuse reflection spectra in the range from 450 to 800 nm were measured to quantitatively determine oxygen saturation（ St O2） of blood vessels in dorsal skinfold window chamber.Furthermore,the changes of the average St O2 and vasoconstriction of blood vessels in the region of interest（ ROI） after V-PDT was evaluated when the nude mice breathing either hypoxic,normoxic,or hyperoxic air under normobaric conditions,respectively. The average St O2 and vasoconstriction of blood vessels for normoxic and hyperoxic groups after VPDT was dramatically changed while no significant change was found for those of blood vessels in hypoxic group. No consistent pattern for the changes of St O2 and vasoconstriction during V-PDT treatment can be observed. However,the VPDT-induced change in St O2 was positively correlated with various irradiances.

关 键 词：血管靶向光动力疗法 皮窗模型 漫反射光谱 血氧饱和度 血管收缩率 

分 类 号：Q631[生物学—生物物理学]