NTERA-2的精原干细胞生物学特征鉴定  

NTERA-2 cells with Characteristics of Spermatogonial Stem Cells

作　　者：王姿力[1] 周勇华[1] 罗超[1] 莫艳秀[1,2] 肖亚梅[1] 

机构地区：[1]湖南师范大学生命科学学院教育部蛋白质化学和鱼类发育生物学重点实验室,湖南长沙410081 [2]湘南学院基础医学部,湖南郴州423000

出　　处：《激光生物学报》2015年第4期364-367,共4页Acta Laser Biology Sinica

基　　金：国家自然科学基金(31172399)资助项目;湖南省教育厅项目资助(15C1274);湖南省研究生科研创新项目资助(CX2013B212)

摘　　要：本研究通过免疫荧光分析,鉴定NTERA-2细胞是否具有精原干细胞特征。研究结果显示,重要精原干细胞标记分子GFRα-1和DDX4在NTERA-2细胞中均表现为阳性表达,NTERA-2细胞具有精原干细胞的特征。初步探讨了重要细胞信号转换蛋白JNK特异性抑制剂SP600125对NTERA-2细胞生长和增殖的影响,为进一步以NTERA-2细胞为精原干细胞模型开展JNK对生殖细胞发育调节功能的研究奠定基础。This study was to identify the characteristics of NTERA-2 cells to find whether out has it the characteristics of spermatogonial biology. Our results showed that NTERA-2 cells expressed some markers of spermatogonial stem cells, such as GFRct-1 and DDX4. It suggest that NTERA-2 ceils maybe useful as a model for studying spermatogonial stem cell proliferation and differentiation in vitro. With the treatment by SP600125, the proliferation of NTERA - 2 cells is se- riously inhibited. The results are the foundation of further research on the function of JNK in germ cell development.

关 键 词：NTERA-2 精原干细胞 SP600125 免疫荧光技术 

分 类 号：Q492[生物学—生理学]