姜黄素衍生物64PH的抗肿瘤作用  

Anti-tumor effects of curcumin derivative 64PH

作　　者：李雪倩[1] 雷海民[2] 王鹏龙[2] 绪扩[2] 禚映辰 陈敬国[1] 郑巧伟[1] 马文斌[1] 赵雪[1] 许君望[1] 封卫毅[1] 

机构地区：[1]西安交通大学医学院第一附属医院,陕西西安710061 [2]北京中医药大学中药学院,北京100102

出　　处：《中国医院药学杂志》2015年第17期1535-1539,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(编号:81071765;81372379和81073017);北京中医药大学创新团队资助项目(编号:2011-CXTD-15)

摘　　要：目的:探讨姜黄素衍生物64PH的体内外抗肿瘤活性。方法:MTT法检测64PH对小鼠B16黑色素瘤细胞及人HepG2肝癌细胞的体外增殖抑制作用;采用小鼠移植性肿瘤H22观察64PH的体内抑瘤活性,HE染色观察肿瘤血管新生。结果:64PH对B16的IC50分别为10.30μg·ml-1(24 h),3.12μg·ml-1(48 h),2.67μg·ml-1(72 h),对HepG2的IC50分别为5.60μg·ml-1(24 h),7.60μg·ml-1(48 h),5.92μg·ml-1(72 h);低剂量(100 mg·kg-1)、高剂量(300 mg·kg-1)64PH对小鼠H22的抑瘤率分别为26.1%,33.0%,且可明显抑制小鼠H22肿瘤的血管生成。结论:64PH在体内外均具有较好的抗肿瘤活性。OBJECTIVE To investigate the anti-tumor activities of curcumin derivative 64 PH in vitro and in vivo.METHODS Cytotoxicity of 64 PH to B16 melanoma cells and HepG2 hepatoma cells was tested respectively by MTT assay.Effects of 64 PH on H22 tumor growth were observed in mice.Tumor angiogenesis was observed on HE stained paraffin sections under microscope.RESULTS Proliferations of B16 cells and HepG2 cells was inhibited by 64 PHin vitro.IC50 values of B16 were 10.30μg·ml-1（24 h）,3.12μg·ml-1（48 h）,2.67μg·ml-1（72 h）,and IC50 values of HepG2 were 5.60μg·ml-1（24 h）,7.60μg·ml-1（48 h）,5.92μg·ml-1（72 h）,respectively.Inhibitory rates of 64 PH on H22 tumor growth were 26.1%and 33.0%at 100 mg·kg^-1 and300 mg·kg^-1（intraperitoneal injection）respectively in vivo.Blood vessel densities were（11.67±3.14）/field and（9.67±1.37）/field at 100 mg·kg^-1 and300 mg·kg^-1,which was significantly suppressed.CONCLUSION64 PH shows significant anti-tumor activities in vitro and in vivo.

关 键 词：64PH MTT 抑瘤率 血管新生 

分 类 号：R932[医药卫生—生药学]