地黄不同提取工艺中梓醇含量的比较  

Comparison of contents of catalpol in radix rehmanniae by different extraction methods

作　　者：熊辉[1] 朱军[2] 陈琴华[2] 

机构地区：[1]湖北医药学院附属人民医院,湖北十堰442008 [2]湖北医药学院药物分析与筛选研究所,湖北十堰442008

出　　处：《中国医院药学杂志》2015年第17期1610-1612,共3页Chinese Journal of Hospital Pharmacy

基　　金：湖北省自然科学基金指导性项目(编号:2015CFC811)

摘　　要：目的：比较地黄中梓醇提取的不同方法,以优选出梓醇提取的最佳方法,建立含有地黄中梓醇含量测定方法。方法：分别采用超声波提取、溶剂提取、加热回流提取地黄中梓醇,并用HPLC测定梓醇含量。结果：超声波法提取测得地黄中梓醇含量最高,梓醇在40-400μg·ml-1范围内有良好线性关系（r=0.997 6）,平均加样回收率为97.24%。结论：超声法简便,准确,重现性良好,可用于地黄中梓醇含量测定。OBJECTIVE To compare contents of catalpol in radix rehmanniae by different extraction methods and optimize suitable method and establish a method for determination of catalpol in radix rehmanniae using HPLC.METHODS Ultrasonic extraction,solvent extraction and heat under reflux extraction method were adopted to extract catalpol in radix rehmanniae using HPLC.RESULTS Catalpol content was the highest by ultrasonic extraction.Liner range of catalpol was 40-400 g·ml^-1（r=0.997 6）.Mean extraction recovery was 97.24%.CONCLUSION Ultrasonic method is simple,accurate,reproducible,which is suitable for determination of catalpol in radix rehmanniae.

关 键 词：地黄 梓醇 提取方法 

分 类 号：R932[医药卫生—生药学]