UPLC-MS/MS法同时测定尿液中的6种苯丙胺类毒品  

Simultaneous determination of 6 amphetamine-type drugs in urine samples by UPLC-MS/MS

作　　者：李瑶[1,2] 刘荣[3] 张琳[4] 王朝虹[4] 李虹 刘勇[4] 赵蒙[4] 

机构地区：[1]昆明医科大学生物医学工程中心,昆明650500 [2]云南省第一人民医院口腔内科,昆明650031 [3]昆明医科大学第一附属医院急诊科重症监护室,昆明650032 [4]最高人民检察院司法鉴定中心,北京100040 [5]毒品分析及禁毒技术公安部重点实验室,昆明650000

出　　处：《分析试验室》2015年第9期1072-1075,共4页Chinese Journal of Analysis Laboratory

基　　金：公安部科技强警基础工作专项(2013GABJC015)资助

摘　　要：建立了固相萃取-超高效液相色谱-质谱法（SPE-UPLC-MS/MS）联用技术定量测定尿液中的6种苯丙胺类毒品。样品经Oasis HLB柱提取、纯化后,采用电喷雾离子源电离（ESI）、正离子多反应监测（MRM）模式质谱进行定性和定量分析。6种苯丙胺类毒品分别在0.1～10 ng/m L,0.2～20 ng/m L和0.5～50 ng/m L范围线性良好,相关系数不低于0.9993,提取回收率高于85%,RSD小于10.0%。方法可用于尿液中痕量苯丙胺类毒品的分析。A rapid and sensitive method has been developed for simultaneous determination of 6 amphetamine- type drugs in urine samples by solid phase extraction-ultra performance liquid chromatography coupled with mass spectrometry （SPE-UPLC-MS/MS）. The samples were extracted with Oasis HLB solid phase extraction cartridge and analyzed with UPLC-MS/MS system coupled with an electrospray ionization （ESI） source. The samples were measured in the mode of electrospray positive ionization （ESI ＋ ） and multiple reaction monitoring （MRM）. 6 amphetamine-type drugs exhibited a good linearity in the range of 0. 1 - 10 ng/mL,0.2 -20 ng/mL and 0.5 - 50 ng/mL, respectively, with correlation coefficients over 0. 9993. The recoveries were above 85% with RSD less than 10. 0%. The method was proved to be simple, rapid, sensitive and accurate for the trace determination of amphetamine-type drugs in urine sample.

关 键 词：苯丙胺 固相萃取 UPLC-MS/MS 尿液 

分 类 号：R917[医药卫生—药物分析学]