50Hz 1.8mT正弦交变电磁场通过调节成骨细胞PGE_2分泌影响Opg/Rankl的基因表达  

50Hz 1.8 mT Sinusoidal Electromagnetic Fields Increases Opg / Rankl mRNA Expression Ratio by Regulating PGE_2 Secretion

作　　者：方清清 李志忠[1] 陈克明[2] 周建[2] 闫娟丽[1] 石文贵[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,兰州730050 [2]兰州军区兰州总医院骨科研究所,兰州730050

出　　处：《中国生物化学与分子生物学报》2015年第9期983-988,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.81270963)~~

摘　　要：前列腺素E2(prostaglandin E2,PGE2)作为细胞因子,在骨代谢中扮演重要角色.它通过刺激成骨细胞核因子κB受体活化因子配基(receptor activator of nuclear factor kappa B ligand,RANKL)表达,促进破骨细胞的分化成熟.然而,其是否参与了电磁场调节骨代谢仍不清楚.PGE2的生物合成受到环加氧酶(cyclooxygenase,COX)的调节.在细胞中存在2种不同的环加氧酶,COX-1和COX-2.其中,COX-2是引起PGE2分泌增加的主要原因.其活性受到细胞核因子κB(nuclear factor kappa B,NF-κB)的调节.本文通过检测体外培养成骨细胞PGE2分泌,COX-2蛋白表达以及Cox-2、Opg、Rankl和Nf-κb基因表达发现,经50 Hz 1.8 m T正弦交变电磁场(sinusoidal electromagnetic fields,SEMFs)处理后,由COX-2介导的PGE2分泌以及cox-2、Nf-κb的基因表达皆下调,但Nf-κb的变化先于cox-2的变化,而opg/rankl基因表达则恰恰相反,说明电磁场通过抑制Nf-κb的转录降低由COX-2介导的PGE2的分泌,进而降低对Rankl表达的刺激作用,抑制破骨细胞的分化成熟.The cytokine prostaglandin E2（ PGE2） plays an important role in bone formation and absorption. The synthesis of PGE2 depends on the cyclooxygenase（ COX）,which has two isoenzymes of COX-1 and COX-2. Regulated by nuclear factor kappa B（ NF-κB）,COX-2 induced the secretion of PGE2. PGE2 promotes the differentiation and maturation of osteoclasts through stimulating the expression of receptor activator of nuclear factor kappa B ligands（ RANKL）. It is still unclear whether PGE2 was involved in bone metabolism with the exposure of electromagnetic fields. The present study investigated the effects of 50 Hz 1. 8 mT sinusoidal electromagnetic fields（ SEMFs） on the secretion of PGE2. The expression of Cox-2,Opg,Rankl and Nf-κb in osteoblasts were determined,as well as the protein levels of COX-2 in vitro. The results indicated that the secreted PGE2 and mRNA levels of Nf-κb,Cox-2 were decreased. The Opg / Rankl mRNA expression ratio was significantly increased. It suggested that the treatments of 50 Hz 1. 8 mT SEMFs inhibited the gene expression of Nf-κb,and COX-2 activity todecrease PGE2 secretion,possibly involved Rankl to affect the formation and maturation of osteoclasts.

关 键 词：正弦交变电磁场 核因子ΚB 环加氧酶-2 前列腺素E2 细胞核因子κB受体活化因子配基 

分 类 号：R318[医药卫生—生物医学工程]