多种挥发性有机物对小鼠睾丸组织相关酶及激素的影响  

Toxic effects of mixture of volatile organic compounds on mice testis related enzymes and hormones

作　　者：郝莉[1] 贺金奖[2] 张磊[1] 郭建峰[3] 齐娜娜[3] 杨章萍[1] 张旭慧[1] 杨洛贤[1] 张增利[3] 

机构地区：[1] 杭州市疾病预防控制中心,310021 [2] 上海出入境检验检疫局 [3] 苏州大学公共卫生学院

出　　处：《中华劳动卫生职业病杂志》2015年第9期682-684,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：杭州市卫生科技计划（2011A056）

摘　　要：目的 研究多种挥发性有机物（VOCs）对小鼠睾丸组织相关酶及激素的影响.方法 急性毒性试验确定VOCs的LD50后,选择清洁级昆明种纯系雄性小鼠40只,分为1/8 LD50 VOCs染毒组、1/4 LD50VOCs染毒组、1/2 LD50 VOCs染毒组、环磷酰胺（60 mg/kg）阳性对照组和茶油阴性对照组,每组8只,连续腹腔注射5d,应用ELISA法测定小鼠睾丸组织中激素睾酮（T）、雌二醇（E2）、卵泡刺激素（FSH）、促黄体生成素（LH）水平,同步测定乳酸脱氢酶（LDH）、γ-谷氨酸转肽酶（γ-GT）、酸性磷酸酶（ACP）、葡萄糖-6-磷酸脱氢酶（G-6-PD）的活力.结果 与阴性对照组比较,1/8LD50 VOCs染毒组小鼠睾丸系数明显增高,差异有统计学意义（P＜0.05）.各染毒组的睾丸标志酶活力随着染毒剂量的增加呈现下降趋势,与阴性对照组比较,差异有统计学意义（P＜0.05）.各染毒组的睾丸性激素水平随着染毒剂量的增加呈现下降趋势,与阴性对照组比较,差异有统计学意义（P＜0.05）.结论 VOCs对雄性小鼠睾丸具有明显的毒性,可影响其睾丸性激素水平和酶活力.Objective To evaluate the toxic effects of mixture of volatile organic compounds（VOCs） on Mice Testis related enzymes and hormones.Methods After determining the median lethal dose （LD50） of VOCs using the acute toxicity test,40 male clean inbred Kunming mice were assigned to 1/8 LD50 VOCs exposure group,1/4 LD50 VOCs exposure group,and 1/2 LD50 VOCs exposure group,as well as positive control group with cyclophosphamide （60 mg/kg） and negative control group with tea oil,with 8 mice in each group.The mice were intraperitoneally injected with respective agents for 5 days.The levels of testis testosterone,estradiol,follicle stimulating hormone,and luteinizing hormone were determined by ELISA.Meanwhile,the activity of testicular marked enzymes such as lactate dehydrogenase,gamma-glutamyl transpeptidase,acid phosphatase,and glucose-6-phosphate dehydrogenase were examined.Results Compared with the negative control group,the 1/8 LD50 exposure group had a significantly increased testis coefficient （P〈0.05）.Both the activity of testicular marked enzymes and the levels of testicular sex hormones in all exposure groups showed significant downward trends with increasing VOC doses compared with those in the negative control group （P〈0.05）.Conclusion VOCs have obvious toxicity to mouse testis by changing the levels of testicular sex hormones and the activity of testicular marked enzymes.

关 键 词：挥发性有机物 睾酮 雌二醇 乳酸脱氢酶 

分 类 号：R135.1[医药卫生—劳动卫生]