姜黄素拮抗百草枯中毒大鼠血清炎症相关细胞因子反应的研究  

Inhibitory effects of curcumin on inflammatory cytokines in rats with paraquat poisoning

作　　者：李红军[1] 刘宝南 李鹏[3] 冯玲吉 马海红[5] 轩诗孟 曹永芝[6] 

机构地区：[1] 山东大学附属省立医院,济南250021 [2] 济南市第六人民医院 [3] 山东省职业卫生与职业病防治研究院 [4] 山东省五莲县人民医院 [5] 山东大学齐鲁医院 [6] 山东大学附属生殖医院科教部

出　　处：《中华劳动卫生职业病杂志》2015年第9期689-692,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：山东省科技发展计划项目（2014GSF118162）

摘　　要：目的 探讨姜黄素在不同治疗条件下对PQ中毒大鼠血清炎症相关细胞因子的影响.方法 将50只清洁级8周龄Wistar雄性大鼠随机分为高剂量（1 000 mg/kg）姜黄素＋常规治疗组、低剂量（500 mg/kg）姜黄素＋常规治疗组、单独高剂量（1 000 mg/kg）姜黄素治疗组、PQ单纯染毒组和空白对照组,分别在染毒后第1、3、5、7、14和21天检测大鼠血清中转化生长因子β1（TGF-β1）、肿瘤坏死因子（TNF-α）、白细胞介素6（IL-6）含量,HE染色观察肺组织病理变化.结果 与空白对照组相比,高剂量姜黄素＋常规治疗组、低剂量姜黄素＋常规治疗组、单独高剂量姜黄素治疗组以及PQ单纯染毒组大鼠血清中TGF-β1、TNF-α和IL-6含量明显升高,差异均有统计学意义（P＜0.05）,其高峰出现在染毒后第14天.与PQ单纯染毒组比较,单独高剂量姜黄素治疗组大鼠血清中TGF-β1、TNF-α和IL-6含量明显降低,差异有统计学意义（P＜0.05）.染毒后第21天,高剂量姜黄素＋常规治疗组大鼠血清中TGF-β1、TNF-α和IL-6含量与低剂量姜黄素＋常规治疗组比较,差异无统计学意义（P＞0.05）.肺组织HE染色结果显示,高剂量姜黄素＋常规治疗组、低剂量姜黄素＋常规治疗组、单独高剂量姜黄素治疗组、PQ单纯染毒组大鼠第21天大量肺纤维化形成,但单独高剂量姜黄素治疗组明显轻于PQ单纯染毒组.结论 姜黄素可明显降低大鼠炎症反应水平,改善肺组织病理变化,抑制并延缓机体损伤的发生发展.Objective To explore the mechanism of paraquat （PQ） poisoning and to observe the changes in inflammatory cytokines in PQ-exposed rats treated in different ways.Methods Fifty 8-week-old clean male Wistar rats were randomly divided into high-dose curcumin plus conventional treatment group,low-dose curcumin plus conventional treatment group,high-dose curcumin group,PQ poisoning group,and blank control group.On days 1,3,5,7,14,and 21 after PQ exposure,serum levels of transforming growth factor-β1 （TGF-β1）,tumor necrosis factor-α （TNF-α）,and interleukin-6 （IL-6） were measured.The pathological changes in lung tissue were evaluated by HE staining.Results Compared with the blank control group,the high-dose curcumin plus conventional treatment group,low-dose curcumin plus conventional treatment group,high-dose curcumin group,and PQ poisoning group had significantly increased serum levels of TGF-β1,TNF-α,and IL-6 （P〈0.05）,and the three cytokines in each group reached peak levels on day 14 after exposure.Compared with the PQ poisoning group,the high-dose curcumin group had significantly reduced serum levels ofTGF-β1,TNF-α,and IL-6 （P〈0.05）.On day 21 after exposure,there were no significant differences in serum levels of TGF-β1,TNF-α,and IL-6 between the high-dose curcumin plus conventional treatment group and the low-dose curcumin plus conventional treatment group （P〉0.05）.The HE staining revealed alveolar inflammatory changes on days 1 ～7 and massive pulmonary fibrosis on days 14～21 in the high-dose curcumin plus conventional treatment group,low-dose curcumin plus conventional treatment group,high-dose curcumin group,and PQ poisoning group,but the above changes were milder in the high-dose curcumin group than in the PQ poisoning group.Conclusion For rats with PQ poisoning,curcumin can significantly reduce inflammatory response and pathological changes in lung tissue and inhibit and delay the development and progression of body injury.

关 键 词：大鼠 姜黄素 百草枯 细胞因子 

分 类 号：R285.5[医药卫生—中药学]