不同时间头针对大脑中动脉梗塞大鼠缺血局部脑皮质微循环状态的影响  

Effect of Scalp Needling at Different Time Points on Focal Cerebral Microcirculation in Rats with Ischemia Induced by Middle Cerebral Artery Occlusion

作　　者：梁超 王静芝[2] 姜涛 

机构地区：[1]海口市中医医院,海南海口570216 [2]湖北中医药大学,湖北武汉430000

出　　处：《广州中医药大学学报》2015年第5期874-879,972,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：海南省自然科学基金资助项目(编号:20158371)

摘　　要：【目的】观察不同时间头针治疗对局灶性脑缺血再灌注大鼠缺血局部脑皮质微循环状态改变的影响。【方法】选用100只SD大鼠随机分为正常对照组、假手术组、模型组(分别按缺血再灌注12、24、48、72 h分为4个亚组),头针组(分别按缺血再灌注12、24、48、72 h加头针分为4个亚组)。除正常对照组外,假手术组仅分离血管而不插线栓,模型组、各治疗组大鼠均采用大脑中动脉线栓法复制大脑中动脉梗塞(MCAO)脑缺血再灌注模型,治疗组采用电针头穴顶颞前斜线、顶颞后斜线进行干预,其余组不予以任何干预。比较神经功能缺损评分(NSS)观察急性脑缺血再灌注各时间点头针对模型大鼠神经功能障碍的影响;采用激光多普勒血流仪检测大鼠实验前,缺血再灌注12、24、48、72 h后局部脑组织血流量的变化;采用免疫荧光染色法检测缺血局部脑皮质微血管内皮细胞数目变化情况;对各组大鼠试验后缺血局部脑皮质血流值(r CBF)与微血管内皮细胞增殖数目进行Pearson相关性统计分析。【结果】头针组大鼠的NSS均显著低于模型组(P<0.05或P<0.01)。实验前,正常对照组、假手术组、模型组和头针组大鼠的缺血脑皮质r CBF比较差异均无统计学意义(P>0.05);除再灌注24 h外,头针组较模型组在各时间点的r CBF显著增多(P<0.05或P<0.01);头针组再灌注24、48、72 h的微血管内皮细胞增殖数目均显著多于模型组的(P<0.05或P<0.01)。模型组和头针组大鼠实验后局部脑血流量的变化与微血管内皮细胞增殖数目呈正相关。【结论】头针治疗能明显改善脑缺血再灌注后脑皮质的微循环状态,提高脑缺血再灌注大鼠局部脑皮质血流量,增加缺血再灌注后局部脑皮质微血管内皮细胞的数量,促进缺血组织的修复。Objective To observe the effect of scalp needling at different time points on focal cerebral microcirculation in rats with focal ischemia and reperfusion （ I-R）. Methods One hundred SD rats were randomized into normal group, sham operation group, model group and scalp needling group. The model group and scalp needling group were divided I-R 12, 24, 48 and 72 h subgroups according to the time of ischemia and reperfusion, 10 rats in each subgroup. The normal group had no oper ation, sham operation only had the operation for vessel isolation, and model group and scalp needling group received middle cerebral artery occlusion with thread. Scalp needling group also had electro-acupuncture on scalp points along vertex and temple anterior linea oblique and posterior linea oblique. And then we observed the neurological severity scores （NSS） and detected the cerebral blood flow （ CBF） in the focal ischemic brain with laser Doppler blood stream meter before I-R and 12, 24, 48 and 72 h after I-R. Immunofluorescence method was used for counting the focal ischemic cerebral microvascular endothelial cells, and then the correlation of cerebral microvascular endothelial cells count with the cerebral blood flow volume was evaluated with Pearson correlation analysis. Results NSS in scalp needling group was lower than that in the model group （P〈0.05 or P〈0.01）. Before I-R, the difference of CBF among the three groups was insignificant （ P〉0.05）. Except for reperfusion for 24 hours, CBF in scalp needling group was higher than that in the model group at different time points （ P〈0.05 or P〈0.01）. Microvascular endothelial cells count in scalp needling group was higher than that in the model group after reperfusion for 24, 48 and 72 hours （ P〈0.05 or P〈0.01）. In the model group and scalp needling group, cerebral blood flow volume was positively correlated with microvascular endothelial cells count. Conclusion Scalp needling shows obvious effect on improving the microcirculation, increasing CBF a

关 键 词：急性脑缺血再灌注/针灸疗法 脑血流量 微血管内皮细胞 脑皮质/病理学 疾病模型 动物 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]