三芪口服液影响单侧肾切除糖尿病大鼠足细胞及裂孔膜相关分子表达研究  

Effect of Sanqi Oral Liquid on Podocytes and Slit Diaphragm-associated Molecules Expression in Diabetes Mellitus Rats After Unilateral Nephrectomy

作　　者：潘伟钰 路艳蒙[2] 梁康礼[1] 聂钊源[3] 范萍[1] 

机构地区：[1]广州医科大学附属第一医院,广东广州510120 [2]南方医科大学分析测试中心,广东广州510515 [3]广州医科大学附属第三医院,广东广州510150

出　　处：《广州中医药大学学报》2015年第5期880-883,973,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81102586)

摘　　要：【目的】观察三芪口服液对单侧肾切除糖尿病(DM)大鼠足细胞及裂孔隔膜蛋白分子(Nephrin、Podocin、CD2AP)表达的影响,探讨其肾脏保护的作用机制。【方法】选用雄性SD大鼠,随机分为假手术组、模型组、中药组,采用以单侧肾切除,配合小剂量链脲佐菌素(STZ,剂量35 mg/kg)腹腔注射方法复制单侧肾切除DM大鼠模型;各组分别给予三芪口服液(剂量为2.5 g·kg-1·d-1)及等容积蒸馏水灌胃,干预8周;测量足细胞密度及足突宽度等变化,应用免疫组织化学技术观察各组大鼠肾组织Nephrin、Podocin、CD2AP蛋白表达改变。【结果】中药组大鼠足细胞密度增加,足突病变较轻;肾组织Nephrin、Podocin、CD2AP蛋白表达较模型组显著增加(P<0.05或P<0.01)。【结论】三芪口服液对单侧肾切除DM大鼠的肾脏保护作用可能与减轻足细胞损伤,调节足细胞Nephrin、Podocin、CD2AP的蛋白表达有关。Objective To observe the effect of Sanqi Oral Liquid on the podocytes and the expression of slit diaphragm-associated molecules （including Nephrin, Podocin and CD2AP proteins） of diabetes mellitus （DM） rats after unilateral nephrectomy, so as to explore its mechanism for protecting renal function. Methods SD male rats were randomized into sham operation group, model group and Chinese medicine group. The experimental diabetes mellitus （ DM） model was given unilateral nephrectomy and intraperitoneal injection of 35 mg/kg of streptozocin （STZ）. And then the model rats were given intragastric administration of Sanqi Oral Liquid （2.5 g· kg^-1·d^-1） and the same volume distilled water respectively for 8 weeks. After treatment, the density of podocytes and the podocytic foot process width of different groups were measured. Immunohistochemistry was used to observe the changes of the expression of Nephrin, Podocin and CD2AP in renal tissues of different groups. Results After treatment with Sanqi Oral Liquid, the density of podocytes was increased, the foot process lesion was relieved, and the expression levels of Nephrin, Podocin and CD2AP proteins were increased （P〈0.05 or P〈0.01 as compared with those in the model group）. Conclusion The protective mechanism of Sanqi Oral Liquid for renal function of unilateral nephrectomy DM rats is possibly related with the alleviation of podocyte injury and with the regulation of the expression of Nephrin, Podocin and CD2AP proteins in podocytes.

关 键 词：三芪口服液/药理学 糖尿病/中药疗法 足细胞/超微结构 裂孔膜相关分子 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]