肾气丸对庆大霉素致聋豚鼠耳蜗毛细胞钙通道蛋白CaV1.3的影响  

Effect of Shenqi Wan on Calcium Channel Protein Ca V1.3 in Cochlear Hair Cells of Guinea Pigs with Ototoxic Deafness Induced by Gentamicin

作　　者：李欣怡[1] 王枫[1] 管燕平[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《广州中医药大学学报》2015年第5期896-898,971,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：浙江省教育厅科研基金项目(编号:Y201224225);浙江中医药大学校级科研基金项目(编号:20132210)

摘　　要：【目的】观察金匮肾气丸对庆大霉素(gentamycin,GM)中毒性耳聋豚鼠耳蜗毛细胞钙离子通道蛋白Ca V1.3表达的影响。【方法】将40只健康级白色红目豚鼠随机分为正常组,模型组,金匮肾气丸高、中、低剂量组,模型组豚鼠每日肌肉注射庆大霉素120 mg/kg连续10 d,金匮肾气丸高、中、低剂量组豚鼠分别每日肌肉注射庆大霉素120 mg/kg,同时给予剂量为20.3、13.5、6.08 g·kg-1·d-1的金匮肾气丸水煎剂灌胃给药连续10 d。采用免疫组织化学法检测各组耳蜗毛细胞Ca V1.3阳性表达平均光密度值。【结果】各组豚鼠毛细胞Ca V1.3钙离子通道蛋白平均光密度值比较,模型组较正常组显著降低(P<0.01),中药高剂量组较模型组显著升高,差异有统计学意义(P<0.05)。【结论】金匮肾气丸对GM所致的豚鼠耳中毒具有一定的防治作用,可保护听功能。Objective To observe the effect of Jingui Shenqi Wan （JSW） on the content of calcium channel protein CaV1.3 of guinea pigs with ototoxic deafness induced by gentamicin （GM）. Methods Forty healthy guinea pigs were randomly divided into normal group, model group and high-, middle-, and low-dose JSW treatment groups. The model group was given intramuscular injection of GM （ 120 mg/kg） per day for 10 continuous days. The high-, middle-, and low-dose JSW treatment groups were given intramuscular injection of GM （120 mg/kg） and intragastric administration of JSW in the dosage of 20.3, 13.5, 6.08 g·kg^-1·d^-1 respectively per day for 10 days. Immuno histochemistry was used to detect the mean optical density value of CaV1.3 expression in cochlear hair cells. Results There were significant differences of the optical density value of CaV1.3 in cochlear hair cells between the model group and normal group （P〈0.01）, and between the model group and high-dose JSW treatment group （P〈0.05）. Conclusion JSW has certain effect on preventing and treating guinea pig with ototoxic deafness induced by GM, thus to protect the hearing function.

关 键 词：肾气丸/药理学 耳聋/中药疗法 耳蜗毛细胞/病理学 疾病模型 动物 豚鼠 

分 类 号：R285.5[医药卫生—中药学]