蒲黄总黄酮对棕榈酸诱导损伤INS-1胰岛β细胞的干预作用  

Intervention Effects of Pollen Typhae Total Flavone on INS-1 Pancreatic β Cells Damage Induced by Palmitic Acid

作　　者：冯晓桃[1] 刘佳[1] 梁宁[2] 

机构地区：[1]广西中医药大学广西中医药科学实验中心,广西南宁530001 [2]广西中医药大学壮医药学院,广西南宁530001

出　　处：《广州中医药大学学报》2015年第5期899-902,913,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81202677);广西自然科学基金资助项目(编号:2014GXNSFAA118264)

摘　　要：【目的】探讨蒲黄总黄酮(PTF)对饱和脂肪酸棕榈酸(PA)诱导损伤INS-1胰岛β细胞的影响。【方法】采用长期PA诱导INS-1胰岛β细胞的方法建立细胞损伤模型,并给予PTF干预处理,采用二甲氧唑黄(XTT)比色法检测细胞活性。【结果】PA呈浓度和时间依赖性损伤INS-1胰岛β细胞活性,而PTF则呈浓度依赖性改善PA对INS-1胰岛β细胞的损伤。此外,PA对INS-1胰岛β细胞损伤的幅度越大,PTF对损伤的改善作用也就越大。【结论】PTF能改善PA长期诱导所致的INS-1胰岛β细胞损伤。Objective To investigate the effects of Pollen Typhae total flavone （PTF） on INS-1 pancreaticβcell damage induced by palmitic acid （ PA） . Methods INS-1 pancreatic β cells were given long-term induction with PA to establish the impaired cell model, and then were intervened with PTF. Cell viability was determined by tetrazolium salt （ XTT） colorimetry. Results PA impaired the viabilities of INS-1 pancreatic β cells in concentration- and time-dependent manner, and PTF improved the impairment of INS-1 pancreatic β cells induced by PA in concentration -dependent manner. Moreover, PTF showed better improvement on the impairment when the INS-1 pancreatic β cells were impaired more seriously by PA. Conclusion PTF has effects on ameliorating the impairment of INS-1 pancreaticβcells induced by PA for long time.

关 键 词：胰岛Β细胞 蒲黄总黄酮/药理学 2型糖尿病/中药疗法 细胞培养 细胞活性 

分 类 号：R285.5[医药卫生—中药学]