寒饮蕴肺证大鼠模型建立方法探索  

Establishment of Rat Model of Syndrome of Cold Fluid Retention in Lung

作　　者：潘宗奇[1] 蔡陈浩[2] 冯贤慧 黄珍[3] 陈务华[4] 

机构地区：[1]广东省中医院肿瘤科,广东广州510120 [2]广州中医药大学第一附属医院,广东广州510405 [3]广州中医药大学第二临床医学院,2008级七年制广东广州510405 [4]广东省第二中医院,广东广州510095

出　　处：《广州中医药大学学报》2015年第5期919-922,972,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局资助项目(编号:20121189)

摘　　要：【目的】比较寒饮蕴肺证大鼠模型3种不同造模方式的差异,探求更简便易行且可靠的造模方法。【方法】将20只大鼠随机分为正常对照组、脂多糖组、烟熏组和冷水浴组,每组5只。除正常对照组外,其余3组采用不同方法建立模型[对目前常用的寒饮蕴肺证大鼠模型的造模方法 (气管滴注脂多糖、烟熏、冷水浴、冰水灌胃)加以组合]。其中脂多糖组予气管滴注脂多糖、烟熏、冷水浴,烟熏组给予烟熏、冷水浴,冷水浴组给予冷水浴、冰水灌胃;各组造模时间均为15 d。实验结束后,观察记录各组大鼠一般情况、体质量、痰液分泌量及肺组织病理检查。【结果】1各造模组较正常对照组大鼠活动减少,体温降低,气促,唇鼻青紫并有分泌物,喷嚏(咳嗽)现象明显。2与正常对照组比较,各造模组大鼠体质量均显著下降(P<0.01),排痰量均显著增多(P<0.05);脂多糖组、烟熏组和冷水浴组体质量及排痰量值虽依次降低,但组间比较差异无统计学意义(P>0.05)。各组肺组织病理检查结果显示:脂多糖组肺损伤程度最重,烟熏组次之,冷水浴组炎症较轻微。【结论】脂多糖组、烟熏组和冷水浴组的造模方法均可成功复制出寒饮蕴肺证大鼠模型;综合多方面因素,以烟熏组造模方法可行性与可靠性更高。Objective To compare different methods for developing rat model of the syndrome of cold fluid retention in lung （ CFRL） , so as to find an easier and more reliable modeling method for CFRL. Methods Twenty rats were divided into 4 groups, namely normal group, lipopolysaccharide （LPS） group, tobacco group, and cold bath group, 5 rats in each group. Lipopolysaccharide group was given intratracheal drip of LPS, tobacco smoking and cold bath, tobacco group was given tobacco smoking and cold bath, and cold bath group was given cold bath and intragastric gavage of cold water. The modeling time in the three groups lasted for 15 days. After the experiment, we compared the general health state, body weight, sputum volume and pathological changes in rats of the four groups. Results （1） Compared with the normal group, activities of rats in the three modeling groups were lowered, body temperature decreased, and the signs of panting, cyanotic nose and lips with excretion, and sneezing （cough） were obvious. （2） Compared with the normal group, the decrease of body weight was obvious （P〈0.01）, expelling sputum volume was increased （P〈0.05） in the model groups. However, the differences among the three model groups had no statistical differences （ P〉0.05）. （ 3） The results of lung tissue slice examination showed that the injury of lung tissue was severe in LPS group, mild in tobacco group and slight in cold bath group. Conclusion Rat model of CFRL has been established successfully in all of the three modeling groups, and in consideration with all respects, the method for tobacco group is the best.

关 键 词：寒饮蕴肺证 疾病模型 动物 大鼠 

分 类 号：R256.1[医药卫生—中医内科学]