“绿宝石”梨组培苗生根培养技术  

Rooting Technique of‘Lyubaoshi'Pear Tissue Culture Seedling

作　　者：孙文英[1] 刘荣宁[1] 张守仕[1] 黄海帆[1] 贺爱莉[1] 杨海蛟[1] 

机构地区：[1]河南农业职业学院园艺园林系,河南郑州451450

出　　处：《北方园艺》2015年第18期116-118,共3页Northern Horticulture

基　　金：河南省教育厅自然科学研究计划资助项目(2010C210001);河南省高等学校青年骨干教师资助计划资助项目(2013GGJS-252)

摘　　要：以"绿宝石"梨试管苗为试材,探讨不同浓度的NAA(0.1、0.2、0.3mg/L)和IBA(0.5、1.0、1.5mg/L)、活性炭(0%、0.1%、0.3%、0.5%)以及培养方法对"绿宝石"梨生根的影响。结果表明:诱导"绿宝石"梨试管苗生根的最佳培养基是1/2MS+1.0mg/L IBA+0.3mg/L NAA+0.3%活性炭(AC)+30g/L蔗糖+5.5g/L琼脂;培养方式为先在含有生长素的培养基中培养7d,然后转入不含生长素的培养基中培养。Using the plantlet of‘Lyubaoshi＇pear as the test material in this experiment,effect of NAA with different concentrations（0.1,0.2,0.3mg/L）and IBA（0.5,1.0,1.5mg/L）and culture method and AC（0%,0.1%,0.3%,0.5%）for rootage were studied.The results showed that the optimal rootage culture medium was 1/2MS＋IBA1.0mg/L＋NAA 0.3mg/L＋AC 0.3%＋sucrose 30g/L＋agar 5.5g/L.The culture method was seven days of culture with auxin,then culture without auxin.

关 键 词：“绿宝石”梨 组培苗 生根 

分 类 号：S661.203.6[农业科学—果树学]