滤液法和沉淀法测定宁夏枸杞果实醇提物中季铵型生物碱的含量  

Determination of Ammonium Alkaloids in Methanol Extract of Lycium barbarum L.by Filtrate and Precipitation Methods

作　　者：马茜[1] 饶建华[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021

出　　处：《北方园艺》2015年第18期142-145,共4页Northern Horticulture

基　　金：国家科技支撑计划子课题资助项目(2009BAI72B03)

摘　　要：以宁夏枸杞为试材,基于季铵型生物碱与雷氏盐的反应原理,采用分光光度法测定宁夏枸杞果实醇提物中季铵型生物碱含量,建立了宁夏枸杞果实醇提物中季铵型生物碱的含量测定方法。结果表明:滤液法与沉淀法的平均回收率分别为97.57%与98.06%,RSD分别为1.02%与1.10%(n=6)。由此看出,滤液法与沉淀法用于季铵型生物碱的含量测定,所得结果基本一致,滤液法操作相对简便。Lycium barbarumL.was used as raw material,and spectrophotometry was used to determine content of ammonium alkaloids in methanol extract of Lycium barbarumL.,based on the reaction of ammonium alkaloids with Remecke salt.The aim was to establish a method for quantitative determination of ammonium alkaloids in methanol extract of Lycium barbarumL.The results showed that the average recovery rate of filtrate and precipitation methods was 97.57%and 98.06%,and relative standard deviation was 1.02%and 1.10%（n=6）,respectively.Conclusion,the results were consistent for determination of ammonium alkaloids by the two methods,and filtrate method was comparatively simple.

关 键 词：宁夏枸杞 季铵型生物碱 雷氏盐 分光光度法 滤液法 沉淀法 

分 类 号：R282.71[医药卫生—中药学]