秦巴山区黑木耳液体种培养基的响应面优化  

Optimization of Liquid Culture Mediums of Auricularia auricular Strain Collected From Qinba Mountains by Response Surface Methodology

作　　者：钱雪婷 陈文强[1,2] 邓百万[1,2] 彭浩[1,2] 解修超[1,2] 陈琰[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723000 [2]陕西省食药用菌工程技术研究中心,陕西汉中723001

出　　处：《北方园艺》2015年第18期157-162,共6页Northern Horticulture

基　　金：陕西省科学技术研究发展计划资助项目(2014K01-16-03);陕西省"13115"科技创新工程计划资助项目(2008ZDGC-04)

摘　　要：以秦巴山区黑木耳菌株‘神农A8’为试材,在单因素试验的基础上,通过Box-Behnken设计对黑木耳液体种培养基进行响应面优化。结果表明:黑木耳液体种培养基最佳碳源、氮源分别是葡萄糖、牛肉粉,葡萄糖浓度、牛肉粉浓度以及KH2PO4浓度对菌丝生物量影响较大,优化得到黑木耳液体种培养基的配方是2.16%葡萄糖、0.48%牛肉粉、15%马铃薯、0.33%KH2PO4、0.1%MgSO4·7H2O,菌丝生物量的预测值为11.11g/L,采用上述优化的黑木耳液体种培养基进行验证性试验,测得菌丝生物量为11.37g/L,与预测值的相对误差为2.34%。因此,说明响应面法优化黑木耳液体种培养基是有效可行的。Taking Auricularia auricular ‘Shennong A8＇collected from Qinba Mountains as materials,on the base of single facter experiments,the liquid culture medium of Auricularia auricular strain was optimized by Box-Behnken design.The results showed that the best carbon source was glucose and the best nitrogen source was beef powder,and the concentration of glucose,beef powder and KH2PO4 were the influential parameters on the mycelial biomass.And the best liquid culture medium was achieved and listed as follows：2.16% glucose,0.48% beef powder,15% potato,0.33% KH2PO4,0.1% MgSO4·7H2O.Under the optimal conditions,the mycelial biomass of Auricularia auricular was 11.37g/L,compared with the theoretical value 11.11g/L,the relative error was 2.34%.So the response surface method is effective and feasible for the optimization of liquid culture mediums of Auricularia auricular strain.

关 键 词：黑木耳 菌丝生物量 液体种培养基 BOX-BEHNKEN设计 

分 类 号：S646.6[农业科学—蔬菜学]