慢性氟中毒大鼠血细胞MGMT与hMLH1基因mRNA的表达  

The Changes of Expression of MGMT and hMLH1 mRNA Levels in the Blood Cells of Chronic Fluorosis Rats

作　　者：王煜蘅[1] 苏艳丽[2] 吴昌学[1] 李毅[1] 官志忠[1,3] 齐晓岚[1] 

机构地区：[1]贵州医科大学分子生物学重点实验室,贵州贵阳550004 [2]贵阳护理职业学院检验系,贵州贵阳550081 [3]贵州医科大学病理学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第10期1013-1015,1023,共4页Journal of Guiyang Medical College

基　　金：国家科技支撑计划项目(2013BAI05B03);国家自然科学基金资助项目(81160335);贵州省科技厅重点项目[黔科合计Z字(2012)4010];贵州省科技厅国际合作项目[黔科合外G字(2011)7014];贵州省科技厅科技计划项目[黔科合LG字(2012)009]

摘　　要：目的:研究慢性氟中毒大鼠血细胞中O6-甲基鸟嘌呤-DNA甲基转移酶基因(MGMT)及错配修复基因(h MLH1)mRNA表达水平。方法:采用饮水加氟法复制20只慢性氟中毒大鼠模型为染氟毒组,另取20只大鼠饮用含氟量<0.5 mg/L自来水为对照组,两组大鼠饲养6月时,观察大鼠氟斑牙的形成,鉴定染氟是否成功,同时采取股动脉放血处死大鼠,提取大鼠血细胞总RNA,实时荧光定量PCR法检测染氟毒组及对照组大鼠MGMT、h MLH1基因mRNA水平。结果:染氟组20只大鼠全出现氟斑牙,表明染氟成功;氟中毒大鼠血液MGMT、h MLH1基因mRNA表达水平低于对照组,差异有统计学意义(P<0.05)。结论:MGMT和h MLH1 mRNA水平降低可能是氟中毒引起DNA损伤的机制之一。Objective： To investigate the effects of chronic fluorosis on the DNA repair gene O^6-meth- yl-guanine-DNA methyl-transferase （MGMT） and human mismatch repair gene（ hMLH1 ） mRNA levels in blood cells. Methods： Establish the model of chronic fluorosis rats by adding sodium fluoride to the drinking water of 20 fluorosis rats, and another 20 rats take the water with fluoride content less than 0. 5 mg/L as control group. After raising them for 6 months then executing them by bleeding femoral artery, collect their arterial blood and extract the RNA, detect mRNA expression level of MGMT, MLH1 gene from each group by real-time fluorescence quantitative PCR. Results： All 20 rats in experiment group showed signs of dental fluorosis, indicating successful fluoride results. The levels of MG- MT and MLH1 mRNA were decreased, the transcription difference between the experimental group and control group had statistic value （P 〈 0.05 ）. Conclusion ： MGMT mRNA and hMLH1 mRNA expres- sion in the experimental group decrease significantly than the control group, and the reduction might be the reason in the mechanics of DNA injury.

关 键 词：O^6-甲基鸟嘌呤-DNA甲基转移酶基因 错配修复基因 氟化物中毒 慢性 大鼠 Sprague-Dawley 模型 动物 

分 类 号：R599.5[医药卫生—内科学] R34-33[医药卫生—临床医学]