外源维生素对高NaCl胁迫下紫花苜蓿种子萌发及恢复性的影响  

Effects of Exogenous Vitamins on Seed Germination and Recovery of Medicago sativa L. Under High NaCl Stress

作　　者：刘艳军[1,2] 范晶[1,2] 韩学珅 李斌[1,2] 张义[1,2] 

机构地区：[1]黑龙江大学农业资源与环境学院 [2]黑龙江大学农业资源利用与环境安全重点实验室,哈尔滨150080

出　　处：《中国农学通报》2015年第26期12-17,共6页Chinese Agricultural Science Bulletin

基　　金：黑龙江大学博士启动基金项目"两型豆knox-I;lfy基因克隆与表达分析"(2006-08);黑龙江大学实验室开放基金项目"外源VPP和VB6对NaCl胁迫下紫花苜蓿种子萌发特性的影响"(14K213);创新训练项目"VC和VB1对NaCl胁迫下紫花苜蓿种子萌发特性的影响"(2014NY14)

摘　　要：为给改进紫花苜蓿在盐碱地区的生产提供依据,笔者研究了14610 mg/L高浓度NaCl胁迫下,外源水溶性维生素(VB1、VB2、VPP、VB6、VH、VB12与VC)对紫花苜蓿种子萌发及恢复萌发性状的影响。结果表明:62.5 mg/L VPP显著提高了恢复萌发后的芽鲜重,1000 mg/L VB6、500 mg/L VB12和1000 mg/L VC促进了紫花苜蓿高盐胁迫下的萌发,但对胁迫减轻后的恢复萌发没有显著影响。250 mg/L VB12对高盐胁迫下紫花苜蓿的萌发及恢复萌发均有显著性改进,使高Na Cl的致死性从19.667%降低到4.667%。VB1、VB2和VH未能有效改进高NaCl胁迫对萌发的抑制效应。试验显示外源VB6、VB12、VC对高NaCl胁迫下紫花苜蓿种子的萌发特性有一定程度的调节作用。In order to provide basis for the production of Medicago sativa L. in saline-alkali soil, the author assessed the effect of exogenous water-soluble vitamins（VB1, VB2, VPP, VB6, VH, VB12 and VC） on the seed germination and recovery of M. sativa under 14610 mg/L NaCl stress. The results showed that 62.5 mg/L VPPsignificantly increased the shoot fresh weight after the recovery germination. 1000 mg/L VB6, 500 mg/L VB12 and 1000 mg/L VC substantially promoted the germination under high salinity stress, but had no significant impact on the recovery germination after the stress was relieved. 250 mg/L VB12 significantly improved both the germination under high NaCl stress and the recovery germination, reduced the mortality rate under high NaCl stress from 19.667% to 4.667%. VB1, VB2 and VH failed to effectively alleviate the inhibitory effect of high NaCl stress on seed germination. The experiment showed that exogenous VB6, VB12 and VC could play a role in regulating the seed germination characteristic of M. sativa under high NaCl stress.

关 键 词：紫花苜蓿 维生素 高NaCl胁迫 种子萌发及恢复 

分 类 号：S551.7[农业科学—作物学]