水蛭新旧提取法制备蛭龙活血通瘀胶囊对脑缺血保护作用的比较研究  

Comparison the protective effect of new and old extraction of Leech in Zhilonghuoxuetongyu capsule on cerebral ischemia in rats

作　　者：杨思进 白雪 蒲清荣 孙琴 赵军宁[4] 王蔚[1,5] 

机构地区：[1]四川医科大学附属中医医院心脑病科,四川泸州646000 [2]四川医科大学附属中医医院制剂室,四川泸州646000 [3]四川医科大学药学部,四川泸州646000 [4]四川省中医药科学院,成都610041 [5]成都中医药大学,成都610075

出　　处：《中国临床药理学杂志》2015年第17期1769-1772,共4页The Chinese Journal of Clinical Pharmacology

基　　金：四川省科技厅应用技术研究和开发基金资助项目(2011SZ0322)

摘　　要：目的比较新、旧水蛭提取法制备蛭龙活血通瘀胶囊的脑缺血保护作用。方法 90只SD大鼠分为9组:假手术组、模型组、对照组、大中小3个剂量A实验组(新胶囊)和B实验组(旧胶囊),每日1次,连续5 d灌胃相应药物后,比较神经功能评分、梗死范围、病理改变,比色法检测丙二醛(MDA)、超氧化物歧化酶(SOD)含量。结果与模型组相比,新、旧胶囊大剂量组,均可使神经功能评分降低;新、旧胶囊的大剂量组和旧胶囊中剂量组,脑缺血干湿重百分比均减少;新胶囊大剂量组和旧胶囊大、中2个剂量组,神经细胞肿胀坏死程度、胞核改变、胶质细胞增生均较模型组轻;新、旧胶囊大剂量组MDA含量均明显降低(P<0.05)。结论新旧提取水蛭法制备蛭龙活血通瘀胶囊对脑缺血均有保护作用,这可能与降低MDA有关。Objective To compare the protection effect of new and old extraction of Leech in Zhilonghuoxuetongyu capsule on the focal cerebral ischemia in rats. Methods Ninety SD rats were randomly divided into 9groups： control group,model group,Buchangnaoxintong group,the new（ A） and old（ B） capsule with large,medium and small dose groups. To fill the stomach in one times a day,for five consecutive days later. And compared the neurological function score,infarction range,pathological changes,and serum methane dicarboxylic aldehyde（ MDA）,superoxide dismutase（ SOD） content were be detected by colorimetric method.Results Compared with model group,the new and old capsule of large dose group were lower the neurological function score. The new and old capsule of large dose and old capsule of medium dose can reduce wet weight,dry weight and percentage of cerebral ischemia. The degree of swelling of the nerve cells necrosis,change of nucleus,proliferation of Glial cell in new capsule of high dose and old capsule of high,middle dose were lighter than model group. The MDA content in the new and old capsule of large dose group were lower,there are significant differences（ P 〈0. 05）. Conclusion The new and old extraction of Leech in Zhilonghuoxuetongyu capsule all have protective effect on focal cerebral ischemia in rats,may be related to reduce the serum MDA.

关 键 词：蛭龙活血通瘀胶囊 脑缺血 丙二醛 超氧化物歧化酶 

分 类 号：R285.5[医药卫生—中药学] R977.9[医药卫生—中医学]