中国虫草一新记录种  

A new record of the genus Ophiocordyceps from China

作　　者：周叶鸣 邹晓[1] 瞿娇娇[1] 梁宗琦[1] 

机构地区：[1]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025

出　　处：《微生物学通报》2015年第9期1710-1716,共7页Microbiology China

基　　金：国家自然科学基金项目(No.31360031);国家自然科学基金重大项目子项目(No.31093440);贵州省科技攻关项目(No.黔科合NY[2013]3034);贵州大学研究生创新基金项目(No.研农2014019)

摘　　要：【目的】对一个寄生鳞翅目幼虫的虫草标本Dxhir140901进行分类鉴定。【方法】采用形态学比较和基于ITS1-5.8S-ITS2r DNA的系统发育与进化网络分析进行鉴定。【结果】形态学观察:标本的分离菌株形态显示其为典型的被毛孢属真菌,具有两型产孢结构:A型产孢细胞柱状,(1.8-6.3)μm×1.8μm;B型产孢细胞锥形,基部柱状,向上逐渐变细无明显颈部,基部宽3-3.8μm,长21-63μm,颈部宽1.8-2.0μm,菌丝末端可直接形成产孢细胞;孢子橘瓣形或卵形,(8.1-10.8)μm×(2.7-5.4)μm,具粘液,黏液层厚1.8-2.7μm。系统发育分析结果显示该菌株与巨针线形虫草Ophiocordyceps macroacicularis聚为一支,支持率为98%,进化网络分析也支持上述结果。【结论】通过与O.macroacicularis的形态比较和分子系统学分析结果,Dxhir140901及其分离株Gzuifr-hir140901为巨针线形虫草Ophiocordyceps macroacicularis S.Ban,T.Sakane&Nakagiri的无性阶段,该种为中国新记录种。[Objective] Identification specimen Dxhirl40901 which parasitized on Lepidopterous larvae. [Methods] The comparison of morphological characteristics and the analysis of phylogenetic tree and the evolution network based on internal transcribed spacer （ITS1-5.SS-ITS2rDNA） sequence were used for identification of this specimen. [Results] Morphological observation： morphological characteristics of the strains Gzuifr-hirl40901 was similar to Hirsutella Pat. It has two types of phialides, A-phialides, column, （1.8-6.3） μm×1.8 μm. B-phialides, columnar base, awl-shape and tapers, width of base 3-3.8 μm, length 21-63 μm, width of neck 1.8-2.0 μm, Phialides formed directly from the end of mycelium. Conidia orange segments or oval, （8.1-10.8） μm×（2.7-5.4） μm, mucus thick 1.8-2.7 μm. From the results of the phylogenetic analysis we can find that the Gzuifr-hirl40901 strains and Ophiocordyceps macroacicularis S. Ban, T. Sakane ＆ Nakagiriin belong to the same clade with a 98% approval rating. Branching sequence analysis supports the same results. [Conclusion] The sample Dxhirl40901 and strains Gzuifr-hirl40901 was a anamorph of O. macroacicularis which is a new record from China.

关 键 词：线形虫草属 无性阶段 分类 蝠蛾幼虫 生物防治 

分 类 号：Q949.32[生物学—植物学]