利用HPCE构建小麦LMW-GS标准图谱初探  

Preliminary Construction of Standard LMW-GS Profile in Common Wheat Using HPCE

作　　者：王玉杰[1] 杨明明[1,2] 董剑[1,2] 高翔[1,2] 赵万春[1,2] 石引刚[1,2] 陈良国[1,2] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]陕西省小麦工程技术研究中心/陕西省小麦新品种培育研究中心,陕西杨凌712100

出　　处：《麦类作物学报》2015年第9期1229-1237,共9页Journal of Triticeae Crops

基　　金："十二五"农村领域国家科技计划项目(2011AA100501);国家现代农业产业技术体系建设专项(CARS-3-2-47)

摘　　要：为给不同小麦品种低分子量谷蛋白亚基(LMW-GS)的鉴定、品质预测提供快速、准确的方法,以黄淮麦区14个普通小麦品种为材料,首先利用SDS-PAGE和分子标记确定LMW-GS组成,然后采用改进的HPCE体系分离LMW-GS,最后通过控制变量法比对小麦品种间相同亚基以判断各LMW-GS亚基在图谱中对应的峰。结果表明,采用SDS-PAGE和分子标记相结合的方法可以更加准确地鉴定小麦LMW-GS;利用HPCE体系获得的小麦LMW-GS图谱具有很高的重复性,重复试验所得小麦LMW-GS图谱中各个峰迁移时间的相对标准偏差均在0.30%以下;通过控制变量法所建立的小麦LMW-GS图谱可以实现Glu-A3a、GluA3c、Glu-A3d、Glu-B3a、Glu-B3d、Glu-B3h的快速鉴定。To provide a fast and accurate way for identification of Low-molecular-weight glutenin sub- units （LMW-GS） and quality prediction from different wheat varieties, 14 common wheat varieties from Huang-Huai wheat region were researched in this study. SDS-PAGE and molecular markers were used to identify the compositions of LMW-GS in 14 wheat varieties ; improved HPCE system was used to separate LMW-GS, controlling variable method was used to identify the corresponding peaks of LMW-GS in profiles by comparing same subunits from different varieties. The results showed that the combination of SDS-PAGE and molecular markers can identify LMW-GS accurately l the repeatability of HPCE profile is high and the relative standard deviation（RSD） of migration time of every peak in duplicate test is less than 0. 3 %the developed HPCE profile can identify GlwA 3a, Glu-A 3c, Glu- A 3d,Glu-B 3a,GlwB 3d,GlwB 3h rapidly.

关 键 词：小麦 低分子量谷蛋白亚基 分子标记 高效毛细管电泳 HPCE图谱构建 

分 类 号：S512.1[农业科学—作物学] S330