金粉蕨属的分子系统学研究——基于5种叶绿体DNA序列片段  

Phylogenetic Analysis of Onychium Based on Five Chloroplast DNA Sequences

作　　者：窦平[1] 杨文利[2] 赵冉冉[1] 张钢民[1] 

机构地区：[1]北京林业大学自然保护区学院,北京100083 [2]河北农业大学园林与旅游学院,保定071000

出　　处：《植物研究》2015年第5期665-671,共7页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31270253);基金委重大国际合作项目(批准号为31110103911)

摘　　要：金粉蕨属(Onychium Kaulfuss)隶属广义凤尾蕨科中的凤尾蕨亚科。迄今为止,该属属下分组及种间界定等仍有诸多问题亟待解决。本研究选取5个叶绿体DNA序列片段(rbc L/atp A/mat K/trn L-trn F/trn G-trnR),采用最大似然法(ML)和贝叶斯法(BI)构建金粉蕨属的系统发育树。结果表明:(1)金粉蕨属的9个成员被分置于两大支上。其中野雉尾金粉蕨(Onychium japonicum(Thunberg)Kunze)、西藏金粉蕨(O.tibeticum Ching&S.K.Wu)、木坪金粉蕨(O.moupinense Ching)、湖北金粉蕨(O.moupinense var.ipii(Ching)K.H.Shing)、栗柄金粉蕨(O.japonicum var.lucidum(D.Don)Christ)、黑足金粉蕨(O.cryptogrammoides Christ)、繁羽金粉蕨(O.plumosum Ching)聚为一支;而金粉蕨(O.siliculosum(Desvaux)C.Christensen)和蚀盖金粉蕨(O.tenuifrons Ching)则聚为另一支,可为该属的属下分组提供分子系统学证据;(2)野雉尾金粉蕨与栗柄金粉蕨在系统树中并没有聚在一起,而是被其它类群分割开来,不支持将后者作为野雉尾金粉蕨的变种,建议将栗柄金粉蕨提升为种的等级;(3)系统树上木坪金粉蕨与湖北金粉蕨的样本聚在一个细支上,支持《中国植物志》将湖北金粉蕨作为木坪金粉蕨变种的分类处理;(4)西藏金粉蕨与野雉尾金粉蕨聚在一起,并得到较高的支持,说明两者的关系近缘。Onychium Kaulfuss, subfamily Pteridoideae of Pteridaceae, has long been in dispute with respect to its sectional circumscription and interspecific definition, We studied the phylogenetic relationships of Onychium based on five chloroplast DNA sequences （rbcL/atpA/matlC/trnL-trnF/trnG-trnR） using Maximum Likelihood （ML） and Bayesian （BI） methods. （1）All species of Onychium were clearly divided into two main groups： Onychium japonicum （Thunberg） Kunze, O. tibeticum Ching ＆ S. K. Wu, O. moupinense Ching, O. moupinense var. ipii（Ching）K. H. Shing, O. japonicum var. lucidum（D. Don）Christ, O. cryptogrammoides Christ and O. plumosum Ching were clustered in a group, while O. siliculosum（Desvaux） C. Christensen and O. tenuifrons Ching were clustered in another group; （2）It provided the molecular evidences for the taxonomy of Onychium at section level. O. japoniucm and O. japoniucm var. lucidum were not united together in the phylogenetic trees, while they were separated by other species, suggesting that O. japoniucm var. lucidum should be treated as a separate species rather than a variety of O. japonicum ; （ 3 ） O. moupinense and O. moupinense var. ipii were gathered in a strongly supported clade, supporting the taxonomic treatment of O. moupinense var. ipii as a variety of O. moupinense in Flora of China ; （4） O. tibeticum and O. japonicum were grouped in a clade with a high bootstrap value with close relation.

关 键 词：金粉蕨属 系统分类 rbcL/atpA/matK/trnL-trnF/trnG-trnR 

分 类 号：S682.35[农业科学—观赏园艺]