中国楤木根皮多糖的微波提取工艺优化及单糖组分分析  

Microwave-assisted Polysaccharide Extraction of Aralia chinensis L. Root and Composition Determination of Monosaccharide

作　　者：王一峰[1] 侯宏红 靳洁[1] 赵博[1] 曹家豪 李筱姣 

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《植物研究》2015年第5期779-785,共7页Bulletin of Botanical Research

基　　金：甘肃省自然科学基金项目(1208RJZA-126);兰州市科技局技术支撑项目(中国楤木多糖化学结构及降血糖研究项目[2013-4-89]);国家中医药管理局(201207002)

摘　　要：采用微波辅助提取中国楤木根皮多糖(ACP),在单因素实验的基础上对影响ACP得率的提取时间、温度、料液比、微波功率4个因素进行正交设计优化,最佳工艺为:提取时间为40 min,温度为50℃,料液比为1∶30(g·m L-1),微波功率400 W,多糖得率为15.189%。粗多糖经Sevage法脱蛋白、透析处理后,进行红外光谱扫描得出ACP具有多糖的特征吸收峰,采用体积排阻色谱-光散射联用法测得ACP的分子量为:Mn=5.259×105g·mol-1。ACP经水解衍生化后用气相色谱—质谱联用(GC-MS)分析发现,ACP是由D-阿拉伯糖、D-葡糖糖、D-半乳糖三种单糖组成,相对摩尔比为:4.67∶76.44∶18.18。We extracted the Aralia chinensis polysaccharide （ACP） from A. chinensis L. root by microwave- assisted technique. With the single factor experiment, we optimized four factors affecting the yield of A. chinensis root including time, temperature, microwave power and solid-liquid ratio by using orthogonal design method. The optimum extracting conditions for polysaccharide yield of 15. 189% was under the ratio of solid to liquid 1：30 （g · mL-1）, 40 min, 50℃, and microwave power of 400 W. Polysaccharides were deproteinized by Sevage method after dialysis treatment, and A. chinensis root had infrared spectral scanning polysaccharide characteristic absorption peaks. Ee measured the molecular weight of A. chinensis root polysaccharides by using SEC-HPLC and I,IS： Mn =4.8 x 105 g · mo1-1. A. chinensis root hydrolysis was derivatized by gas chromatography- mass spectrometry （GC-MS） analysis, and the polysaccharide of A. chinensis were composed of D-arabinose, D-glucose and D-galactose, with the molar ratio of 4.67：76.44：18.18.

关 键 词：微波提取 中国楤木多糖 正交试验 分子量 单糖组分 

分 类 号：Q949.763.2[生物学—植物学]