8-羟基脱氧鸟苷和4-羟基壬烯醛在盆底器官脱垂患者子宫骶韧带中的表达  

Expressions of 8- OHd G and 4- HNE in uterosacral ligament in women with pelvic organ prolapse

作　　者：李洋[1] 洪莉[1] 刘成[1] 洪莎莎[1] 杨青[1] 汤剑明[1] 张琦钒 

机构地区：[1]武汉大学人民医院妇Ⅱ科,湖北武汉430000

出　　处：《中国计划生育和妇产科》2015年第9期25-28,I0002,共5页Chinese Journal of Family Planning & Gynecotokology

基　　金：国家自然科学基金面上项目(项目编号:81270684);湖北省科技支撑计划项目(项目编号:2014BCB045);武汉大学医学部协同创新项目(项目编号:523-266078)

摘　　要：目的探讨盆底器宫脱垂患者子宫骶韧带8一羟基脱氧鸟苷（8-hydroxy-2-deoxyguanosine，8-OHdG）及4-羟基壬烯醛（4-hydroxynonenal，4-HNE）的表达情况，并分析其与盆底器官脱垂（pelvicorganprolapsed，POP）的发病关系。方法选取2012年6月至2014年6月在武汉大学人民医院59例行子宫全切术患者资料，分为Non—POP组20例（对照组）、轻度POP组17例（POPI—Ⅱ）、重度POP组22例（POPⅢ～Ⅳ）3组，于术中切取患者部分子宫骶韧带组织，通过免疫组化的方法检测8-OHdG和4-HNE的表达情况，比较其在各组的差异并作相关性分析。结果8-OHdG和4-HNE在POP组中的表达高于对照组，其中重度POP组最高，各组比较差异有统计学意义（P〈0．05），并且两者在POP组表达呈正相关（P〈0．05）。结论氧化损伤随脱垂程度的加重而增加，子宫骶韧带组织细胞的氧化损伤可能是POP的发病机制之一。Objective To investigate expression of 8 - hydroxy - 2 - deoxyguanosine （8 - OHdG） and （4 - hydroxynonenal, 4 - HNE） in uterosacral ligament of women with pelvic organ prolapsed, and speculate the relationship between oxidative damage and POP. Methods The clinical data of 59 cases underwent total hysterectomy in People＇ s Hospital of Wuhan University form Jan 2012 to Jan 2014 were selected, all cases were divided into 3 groups： control group （20 cases）, POP Ⅰ -Ⅱ group （17 cases）, POPⅢ - Ⅳ group （ 22 eases）, specimen of uterosacral were taken from patients, immunohistochemistry was used to detect 8 - OHdG and 4 - HNE, differences were compared in 3 groups and the correlations were analyzed. Results The level of 8 - OHdG and 4 - HNE in POP groups were higher than in control group, the POPⅢ - Ⅳ group was the highest, the differences had statistical significance among the 3 groups （ P 〈 0.05 ）, the expressions of 8 - OHdG and 4 - HNE were positively correlated in POP groups （ P 〈 0. 05 ）. Conclusion Oxidative damage increases with the severity of prolapse, and oxidative damage in uterosacral ligament may play an important role in pathogenesis of POP.

关 键 词：8-OHDG 4-HNE 盆底器官脱垂 氧化损伤 

分 类 号：R711.23[医药卫生—妇产科学]