吸烟对支气管哮喘大鼠肺组织钙调神经磷酸酶活性的影响  

Effects of cigarette smoking on the activity of calcineurin in asthmatic rat lungs

作　　者：张彩苹[1] 杜永成[1] 许建英[1] 

机构地区：[1]山西医科大学第一医院呼吸科,太原030001

出　　处：《国际呼吸杂志》2015年第17期1299-1303,共5页International Journal of Respiration

基　　金：山西省自然科学基金（2012011037-3）

摘　　要：目的通过测定烟草烟雾暴露后支气管哮喘（简称哮喘）大鼠肺组织中钙调神经磷酸酶（calcineurin，CaN）蛋白、mRNA的表达及CaN活性的变化，探讨吸烟对哮喘CaN表达及活性的影响。方法36只Wistar大鼠随机分为对照组、哮喘组、吸烟哮喘组，制备哮喘和吸烟哮喘大鼠模型。苏木精一伊红染色观察气道炎症情况；图像分析观察支气管管壁厚度；实时定量（Real—time）聚合酶链反应和免疫组织化学法分别测定肺组织中CaNmRNA和蛋白的相对表达量；生物化学法检测大鼠肺组织CaN活性。结果①吸烟哮喘组大鼠气道壁厚度较对照组和哮喘组均明显增加，哮喘组较对照组增加，差异均有统计学意义（P值均〈0．01）；②吸烟哮喘组大鼠肺组织中CaNmRNA和蛋白水平的相对表达及CaN的活性均较对照组和哮喘组高，哮喘组较对照组高，差异均有统计学意义（P值均〈0．05）；③大鼠肺组织CaN活性与大鼠气道壁厚度呈正相关（r=0．652，P〈0．01）。结论吸烟可能通过促进哮喘肺组织CaNmRNA和蛋白的表达及CaN活性的增加而在哮喘气道慢性炎症及气道重塑的形成中起重要作用。Objective To explore the influence of cigarette smoking on the expression and the activity of calcineurin in asthmatic rat lungs by investigating the expression of mRNA and protein and the activity of calcineurin in rat lungs of cigarette smoking combined asthma group. Methods Thirty-six Wistar rats were randomly divided into three groups., control group,asthma group and cigarette smoking combined asthmagroup. The rats were sensitized and challenged with ovalbumin （OVA） or both tobacco smoke and OVA to establish the model. The following parameters were measured .. airway inflammation by HE staining,the bronchial wall thickness （WAt/Pi） by computer-assisted image analysis system. The expression of mRNA and protein and the activity of CaN were detected by quantitative real time PCR, Immunohistochemistry and biochemical method respectively. Results （1）The bronchial wall thickness of the cigarette smoking combined asthma group was significantly increased compared with that of the control group and the asthma group and it was higher in the asthma group than that in the control group （all P 〈0.01）. （2）The expression of CaN mRNA and protein and the activity of CaN in the cigarette smoking combined asthma group were higher than those in the control group and the asthma group,and they were higher in the asthma group than those in the control group （all P 〈 0. 05）. （3） There was a positive correlation between the activity of calcineurin in rat lungs and the bronchial wall thickness （ r = 0. 652, P 〈0.01）. Conclusions Cigarette smoking might play an important part in the development of the chronic airway inflammation and the airway remodeling in asthma by promoting over-expression of CaN mRNA and protein and increasing the the activity of calcineurin in asthmatic rat lungs.

关 键 词：钙调神经磷酸酶 哮喘 吸烟 

分 类 号：R544.1[医药卫生—心血管疾病]