胚胎围植入期超早孕妇女血中早孕因子的分离纯化  

Purification of early pregnancy factor from the sera of pregnant women in peri.implantation

作　　者：阎冰[1] 郝润喜[2] 徐爱萍[1] 闫彩萍[2] 阎来喜[3] 董张兰[4] 房晓芬[1] 孟霞[1] 王建宇[1] 樊晓光[1] 

机构地区：[1]山西医科大学生殖免疫研究室,太原030001 [2]山西医科大学生理学省部共建重点实验室 [3]山西医科大学计算机部 [4]山西医科大学第一附属医院妇产科

出　　处：《国际免疫学杂志》2015年第5期413-418,共6页International Journal of Immunology

基　　金：山西高校科技研究开发自筹项目（20041321）;山西省回国留学基金（200375）;太原市科学技术基金（0503014）

摘　　要：目的分离纯化围植入期超早孕妇女血清中免疫抑制物质——早孕因子（early pregnancy factor，EPF）。方法挑选取超声波监测的排卵受精后1到8d内的山西太原妇女40名，抽取血液20-40mL，RIT检测EPF活性，对收集的400mL胚胎植入期妇女血清，经过DEAESepharose离子交换层析，ConASepharose4B亲和层析以及Heparin Sepharose CL-6B亲和层析进行了EPF的分离纯化。结果受精后1-8天内的妇女血中可检测到EPF。EPF可以被纯化为相对分子质量为18000的单一条带。结论胚胎围植入期超早孕妇女血中EPF蛋白质的分离和纯化，为EPF的研究，尤其是围植入期免疫抑制作用，避免了母亲的免疫系统对半移植物胚胎的排斥的研究提供了理论和应用价值。Objective To purify early pregnancy factor （EPF）from the sera of pregnant women with immunosuppressive conditions. Methods Forty women in Taiyuan, Shanxi, China were chosen to detect ovulation by ultrasonagraphy daily. 20 to 40 mL of blood was collected after 1 to 8 days of fertilization. 400 mL sera from these women were subjected to the detection of EPF activity by rosette inhibition test （ RIT ）. EPT was pu- rified by DEAE Sepharose ion-exchange codumn, Con A sepharose 4b and Heparin Sepharose CL-6B. Result EPF was detected in the blood of pregnant women at 1 to 8 day after fertilization. EPF was purified by chroma- tography with molecular weight of 18 000. Conclusion Purification of EPF in the stage of periimplantation will provide the valuable significance of theoretical and clinical usage for the research of maternal immunologic tolerance of the allogenic fetus.

关 键 词：胚胎围植入期 早孕因子 分离 纯化 

分 类 号：R714[医药卫生—妇产科学]