高效液相色谱法同时测定痰热清注射液中9种有效成分  

Simultaneous HPLC determination of 9 effective components ofTanreqing injection

作　　者：陈永刚[1] 吴世强[1] 马传学[1] 胡冰[1] 

机构地区：[1]江苏省徐州市中心医院药剂科,221009

出　　处：《国际中医中药杂志》2015年第9期817-821,共5页International Journal of Traditional Chinese Medicine

摘　　要：目的 建立同时测定痰热清注射液中新绿原酸、绿原酸、咖啡酸、野黄芩苷、芦丁、异绿原酸A、异绿原酸C、黄芩苷、汉黄芩苷的高效液相色谱法.方法采用Agilent TC-C18（2）色谱柱（250 mm×4.6 mm,5μm）,以乙腈（A）-0.1%甲酸溶液（B）梯度洗脱,流速为1.0 ml/min,柱温30℃,进样量20μl,检测波长280 nm.结果 9种有效成分的线性关系良好,精密度、重复性、稳定性的RSD值均低于2%,加样回收率为99.3%~103.1%.结论 本方法简便、准确、重复性好,可有效控制痰热清注射液的质量.Objective To establish HPLC method for the simultaneous detgrmination of 9 effective components ofTanreqing injection.Methods The Agilent TC-C18 （2） （250 mm×4.6 mm, 5μm） column was used, the mobile phase consisted of acetonitrile （A） acetonitrile-0.1% formic acid aqueous solution （B） in a gradient mode, the flow rate was 1.0 ml/min,the column temperature was 30℃, the injection volume was 10μl, and the detecting wavelength was at 280 nm.Results All the 9 effective components showed a good linear relationship. TheRSDof the precision, reproducibility and stability tests were less than 2%. The average recoveries of the 9 effective components were in the range of 99.3%-103.1%.Conclusion This analysis method is simple, accurate and has a good specificity, which can be suitable for controlling the quality of Tanreqing injection.

关 键 词：高效液相色谱法 痰热清注射液 有效成分 

分 类 号：R286.0[医药卫生—中药学]