代综方对油酸诱导的HepG2细胞内脂质堆积的影响  

Impact of Daizongfang on HepG2 Intracellular Lipid Accumulation Induced by Oleic Acid

作　　者：董菲菲[1,2] 朱晓云[1] 张俊龙[3] 刘喜明[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]复旦大学附属华山医院,上海200040 [3]西交利物浦大学,江苏苏州215123

出　　处：《世界中西医结合杂志》2015年第3期330-334,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：国际合作项目(2010DFA31620);重大新药创制(2011ZX09102-011-08)

摘　　要：目的研究代综方(Dai Zong Fang,DZF)对于体外油酸诱导的HepG2细胞内脂质堆积的作用。方法采用油酸(oleic acid,OA)诱导HepG2细胞形成脂质堆积的肝细胞模型,通过噻唑蓝(MTT)实验检测不同浓度油酸或DZF对HepG2细胞活力的影响,采用BODIPY493/503与苯基吲哚(DAPI)双荧光染色进行胞内脂质堆积的形态学观察,通过MATLAB软件对荧光照片进行数学处理以测量胞内脂质含量。结果 100~800μM的油酸对HepG2细胞存活率的影响无统计学意义(P>0.05),油酸使HepG2细胞发生胞内脂质堆积,与剂量呈正相关;一定剂量的DZF(1 mg/L^20 mg/L)对HepG2细胞存活率的影响无统计学意义(P>0.05),可以显著减少油酸所致的肝细胞胞内脂质含量;10 mg/L的DZF作用4 h起效,作用96 h之内效果稳定;40 mg/L的DZF可降低HepG2细胞存活率(P<0.05),对胞内脂质含量的影响无统计学意义(P>0.05)。结论代综方可以抑制油酸所致HepG2细胞内脂质的堆积。Objective To study the effect of daizongfang on HepG2 intracellular lipid accumulation induced by oleic acid. Methods Oleic acid was used to induce liver cell model of HepG2 intracellular lipid accumulation. MTT experiment was adopted to determine the impacts on HepG2 cell viability with oleic acid of different concentration or daizongfang. DODIPY493 /503 and DAPI double fluorescent staining was adopted for the morphological observation of intracellular lipid accumulation. MATLAB software was used for the mathematical treatment of fluorescent pictures and to measure the content of intracellular lipid. Results Oleic acid 100- 800μm did not brought significant impacts on HepG2 cell survival rate（ P 〉0. 05）. The intracellular lipid accumulation in HepG2 induced by oleic acid was positively relevant with the dose. Daizongfang of certain dose（ 1 mg / L ～ 20 mg / L） did not impact significantly HepG2 survival rate（ P 〉0. 05） and significantly reduced liver cell lipid content induced by oleic acid. Daizongfang 10 mg / L worked in 4 h and the effect was stable in 96 h. Daizongfang 40 mg / L reduced HepG2 survival rate（ P 〈0. 05） and had no significant impact on intracellular lipid content（ P 〉0. 05）. Conclusion Daizongfang inhibits HepG2 intracellular lipid accumulation induced by oleic acid.

关 键 词：代综方 HEPG2 游离脂肪酸 脂代谢异常 BODIPY493/503 

分 类 号：R285.6[医药卫生—中药学]