十四味建中汤对氧惊厥小鼠惊厥潜伏期及脑组织炎症细胞因子含量的影响  

Impacts of Shisiwei Jianzhong Decoction on the Convulsion Latency and the Content of Cytokines in Brain Tissue of the Mice with Oxygen Convulsion

作　　者：雷箴[1] 王国忠[2] 

机构地区：[1]首都医科大学附属北京朝阳医院医学研究中心,北京100020 [2]民航总医院氧疗科,北京100123

出　　处：《世界中西医结合杂志》2015年第7期937-939,共3页World Journal of Integrated Traditional and Western Medicine

摘　　要：目的探讨十四味建中汤对氧惊厥小鼠惊厥潜伏期及脑组织白细胞介素-1β(IL-1β)和白细胞介素-10(IL-10)含量的影响及意义。方法将56只小鼠随机分为7组:氧惊厥6 h组、氧惊厥24 h组、氧惊厥48 h组、十四味建中汤+氧惊厥6 h组(建中汤6 h组)、建中汤24 h组、建中汤48 h组以及正常对照组。建中汤组小鼠在惊厥发生2周前每日灌胃1次十四味建中汤煎剂。将氧惊厥组和建中汤组小鼠置于高压氧(500 k Pa)环境中至惊厥发作,建立氧惊厥小鼠模型。用酶联免疫吸附法检测小鼠脑组织IL-1β和IL-10含量。结果建中汤组小鼠氧惊厥潜伏期与氧惊厥组比较,差异无统计学意义(P>0.05)。氧惊厥24 h组和48 h组小鼠脑组织IL-1β含量均显著高于正常对照组,两组比较,差异有统计学意义(P<0.05);建中汤组24 h组和48 h组小鼠脑组织IL-1β含量均显著低于同时间点氧惊厥组,两组比较,差异有统计学意义(P<0.05)。在同时间点,氧惊厥组、建中汤组和正常对照组之间小鼠脑组织IL-10含量比较,差异无统计学意义(P>0.05)。结论十四味建中汤处理未能显著延长小鼠的氧惊厥潜伏期,但可有效降低氧惊厥后脑组织IL-lβ含量,有助于重建促炎和抗炎细胞因子的平衡,减轻惊厥性脑损伤。Objective To discuss the impacts and significance of shisiwei jianzhong decoction on convulsion latency and the content of IL - 1β and IL - 10 in brain tissue of the mice with oxygen convulsion. Methods Fifty - six mice were randomized into 7 groups,named an oxygen - convulsion 6 h group（6 h group）,a 24 h group,a 48 h group,a shisiwei jianzhong decoction ＋ oxygen - convulsion 6 h group（a therapy 6 h group）,a therapy 24 h group,a therapy 48group and a normal control group. In all of the therapy groups, 2 weeks before convulsion induced by oxygen,shishiwei jianzhong decoction was used for gastric perfusion, once every day. The mice in all of the oxygen convulsion groups and the therapy groups were placed in 500 kPa oxygen environment till the seizure so as to prepare the model of oxygen convulsion. The enzyme - linked immunosorbent assay was used to detect the content of IL - 1β and IL - 10 in brain tissue. Results There was no significant difference in convulsion latency between the therapy groups and the oxygen convulsion groups（P ﹥ 0. 05）. In the 24 h group and the 48 h group,the content of IL - 1β was higher significantly than that in the normal control group,indicating the significant difference（P ﹤ 0. 05）,and that in the therapy 24 h group and the therapy 48 h group was lower significantly than that in the corresponding oxygen convulsion groups of the same time point,indicating the significant difference in the pair comparison（P ﹤ 0. 05）. At the same time point,the difference in the content of IL - 10 was not significant among the oxygen convulsion groups,the therapy groups and the normal control group（P ﹥ 0. 05）. Conclusion Shisiwei jianzhong decoc-tion does not significantly prolong the latency of oxygen convulsion in the mice,but it effectively reduces the content of IL - 1β,benefits the re - balance of proinflammatory cytokine and anti - inflammatory cytokine and alleviates the convulsive brain damage.

关 键 词：氧惊厥 十四味建中汤 脑组织 白细胞介素 - 1β 白细胞介素 - 10 

分 类 号：R285.5[医药卫生—中药学] R720.597[医药卫生—中医学]