辛开苦降方对初发2型糖尿病KKAy小鼠胰腺InR和Bax表达的影响  

Impacts of Xinkai Kujiang Formula on the Expressions of InR and Bax in KKAy Mice of Early T2DM

作　　者：姜旻[1] 张先慧[1] 张艳红[1] 胡照娟[1] 柳红芳[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《世界中西医结合杂志》2015年第7期991-994,998,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目(81072735)

摘　　要：目的观察辛开苦降方对初发2型糖尿病(T2DM)KKAy小鼠胰腺胰岛素受体(InR)和凋亡相关蛋白Bax表达的影响。方法将10周龄雄性初发T2DM KKAy小鼠40只随机分为模型组、马来酸罗格列酮组、辛开苦降低剂量组、辛开苦降高剂量组,每组10只。选取10周龄雄性C57BL/6J小鼠10只为对照组。模型组及正常对照组给予等体积蒸馏水,其余组别分别给予相应的药物灌胃。连续灌胃4周后,测定空腹血糖(FPG)、血清胰岛素(FINS)及胰岛素敏感指数(ISI),应用免疫组织化学法检测小鼠胰腺中InR和Bax的蛋白表达。结果与模型组比较,马来酸罗格列酮组、辛开苦降高剂量组FPG明显降低(P<0.05),各给药组小鼠FINS均降低,ISI均升高(P<0.05)。免疫组化染色,与模型组相比,马来酸罗格列酮组、辛开苦降低剂量组、辛开苦降高剂量组InR阳性细胞均有明显恢复,Bax阳性细胞均有明显减少(P<0.01),其中辛开苦降高剂量组与马来酸罗格列酮组相比无差异(P>0.05),与辛开苦降低剂量组相比差异有统计学意义(P<0.01)。结论辛开苦降方可以使初发T2DM KKAy小鼠胰腺中InR的表达增加,Bax表达减少,提示其改善胰岛自身胰岛素抵抗、抑制胰岛细胞凋亡,且疗效与剂量相关。Objective To observe the impacts of xinkai kujiang formula on the expressions of InR and apoptosis - related protein Bax in KKAy mice of early type 2 diabetes mellitus（T2DM）. Methods Forty KKAy male mice of early T2DM,aged 10 weeks were randomized into a model group,a rosiglitazone maleate group,a xinkai kujiang formula low dose group（low dose group）and a xinkai kujiang formula high dose group （high dose group）,10 mice in each one. Additionally,10 C57BL/ 6J male mice,aged 10 weeks were selected as a control group. The distilled water of same volume was applied in the model group and the normal control group. The medical lavage was given in the rest groups accordingly. After the continuous lavage for 4 weeks, FPG,FINS and ISI were determined. IHC method was adopted to determine the protein expressions of InR and Bax. Results Compared with the model group,FPG was reduced apparently in the rosiglitazone maleate group and the high dose group（P ﹤ 0. 05）. FINS was reduced and ISI was increased in the mice of each med-ication group. Regarding IHC staining,compared with the model group,InR positive cell was recovered appar-ently and Bax positive cell was reduced apparently in the rosiglitazone maleate group,the low dose group and the high dose group（P ﹤ 0. 01）. The differences were not significant in the high dose group as compared with the rosiglitazone maleate group（P ﹥ 0. 05）,but were significant as compared with the low dose group（P ﹤0. 01）. Conclusion Xinkai kujiang formula increases InR expression and reduces Bax expression in the pancreas in KKAy mice of early T2DM. It is suggested that xinkai kujiang formula improves in insulin resist-ance and inhibits pancreatic apoptosis and the efficacy is related to the dose.

关 键 词：2 型糖尿病 辛开苦降方 KKAy 小鼠 胰腺 胰岛素抵抗 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]