高效液相法测定丹参口服液中丹参素钠和原儿茶醛的含量  

HPLC in Measuring the Content of Danshensu Sodium and Protocatechualdehyde in Danshen Oral Liquid

作　　者：姚静[1] 张超[2] 

机构地区：[1]河南中医学院第一附属医院,河南郑州450000 [2]河南中医学院,河南郑州450008

出　　处：《世界中西医结合杂志》2015年第4期502-504,共3页World Journal of Integrated Traditional and Western Medicine

摘　　要：目的建立高效液相法测定丹参口服液中丹参素钠和原儿茶醛的含量控制方法。方法HPLC,C18柱,甲醇-1%醋酸(1∶7)为流动相;检测波长为280 nm;流速1.0 ml/min。结果样品中丹参素钠和原儿茶醛分离完全,阴性供试品无干扰,丹参素钠在0.478 4~2.392 0μg(相当于丹参素0.430 5μg^2.152 8μg)范围内,进样量与峰面积积分值之间线性关系良好;原儿茶醛在0.096μg^0.480μg范围内,进样量与峰面积积分值之间线性关系良好。平均回收率分别为99.95%、99.21%,RSD为1.93%、2.40%(n=5)。结论该方法操作简便、可行、重现性好。Objective To establish a standard in measuring the content of Danshensu sodium and Protocatechualdehyde in Danshen Oral Liquid. Methods HPLC,C18 Column,methyl alcohol（1%）and acetic acid were setup as mobile phase. Detected wavelength was 280 nm,flow velocity was 1. 0 ml/min. Results Separating Danshensu sodium and Protocatechualdehyde and eliminating interference showed Danshensu sodi-um in range of 0. 478 4-2. 392 0 μg（equivalent to Danshensu 0. 430 5μg-2. 152 8 μg）. Sample size and peak area were in good linear relationship. Protocatechualdehyde was in range of 0. 096μg-0. 480 μg. Sam-ple size and peak area were in good linear relationship. Average recovery rate was 99. 95% and 99. 21% re-spectively. RSD was 1. 93% and 2. 40%（n=5）. Conclusion This method is easy to operate and enjoys good repeatability.

关 键 词：丹参口服液 丹参素钠 原儿茶醛 高效液相色谱法 含量测定 

分 类 号：R285.5[医药卫生—中药学]