益气通脉胶囊对脑缺血大鼠脑血管内皮的影响  

Effects of Yiqitongmai Capsule on Cerebral Microvessels of Rats with Cerebral Ischemic Injury

作　　者：彭涛 王永辉[2] 冯振宇 周然[2] 

机构地区：[1]山西省中西医结合医院,山西太原030013 [2]山西中医学院,山西太原030024

出　　处：《世界中西医结合杂志》2015年第4期560-562,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：山西省国际科技合作项目(2013081065-1);山西省回国留学人员科研资助项目(2014-088)

摘　　要：目的观察益气通脉胶囊对脑缺血损伤后大鼠脑微血管内皮细胞的r-谷氨酰转移酶(γ-GT)活性和血清血管内皮细胞生长因子(VEGF)表达的影响。方法大鼠采用反复夹闭双侧颈总动脉结合硝普钠降压法复制SD大鼠拟血管性痴呆模型。造模后选存活大鼠30只随机分为模型组、脑心通组和益气通脉胶囊组高中低剂量组,并设空白对照组。在给药第2天和第7天时尾静脉取血,分离血清,测定VEGF含量。连续给药20 d后分离大鼠大脑皮质的微血管内皮细胞,提取总蛋白,测定γ-GT的活性。结果微血管内皮细胞γ-GT的活性:给药20 d后,益气通脉胶囊中低剂量组大鼠的γ-GT的活性明显高于模型组。血清VEGF的含量:益气通脉胶囊各组的VEGF的含量在给药2 d时与模型组相比差异无统计学意义,在给药7 d时均明显高于模型组。结论益气通脉胶囊对脑缺血所致大鼠血脑屏障的损伤具有修复功能。Objective To observe the effects of Yiqitongmai Capsule on the activity of Gamma Glutamyl Transpeptidase（γ - GT ）in cerebral microvessels and the expression of Vascular Endothelial Cell Growth Factor（ VEGF）in serum of rats with cerebral ischemic injury. Methods Healthy SD rats were randomized into control group and vascular dementia models,which were duplicated by repeated clamping of bilateral common carotid artery combined with sodium nitropruside depressing. Then models were divided into 5 groups：model group,Naoxintong（ NXT）group and experimental groups（ Yiqitongmai Capsule）including high, medium and low dose. On the second and sevenrth day of experiment,the concentrations of serum VEGF in rat were measured. After 20 days of continuous administration,cerebral cortices of rat were dissected and endothelia-enriched fractions were depleted,then extract the total protein and detect the activity of γ-GT. Results （1）The activities of γ-GT in the Yiqitongmai Capsule groups（ low dose and medium dose）were higher than that in the model after 20 days of administration.（2）The concentrations of serum VEGF of all the experimental groups was higher than those of the model after 7 day of administration,while they seem to be similar at the beginning. Conclusions Yiqitongmai Capsule is effective on the repairing cerebral microves-sels in rats with cerebral ischemic injury.

关 键 词：脑缺血 血管内皮细胞 R-谷氨酰转移酶 血清血管内皮细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]