雷公藤红素对AGS细胞迁移的影响  

Effect of Celastrol on AGS Cell Migration

作　　者：杜娜[1] 李健[2] 刘福生[1] 刘泽洲[2] 许可嘉 张寅[1] 刘婷[1] 苏泽琦[1] 丁霞[3] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学基础医学院,北京100029 [3]北京中医药大学医疗处,北京100029

出　　处：《世界中西医结合杂志》2015年第5期623-626,共4页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(91129714;81270466);高等学校博士学科点专项科研基金(20120013110014);国家国际科技合作专项(2009DFA31010)

摘　　要：目的观察不同浓度雷公藤红素在不同时间对AGS细胞形态及细胞迁移的影响,探索雷公藤红素对AGS细胞迁移较佳抑制作用浓度及时间。方法将AGS细胞铺于6孔板并培养,待贴壁细胞密度达到70%~80%后,采用划痕法制造细胞迁移模型,分为DMSO及雷公藤红素5μM、1μM、0.5μM、0.1μM、0.01μM浓度组,并分别于0 h、6 h、12 h、24 h观察细胞的形态及细胞的迁移情况,且拍照记录,计算细胞迁移速度及细胞迁移抑制率并进行各组之间的比较。结果高浓度雷公藤红素使AGS细胞形态发生改变;雷公藤红素能够抑制AGS细胞迁移,且终浓度为1μM时抑制作用最明显。同一浓度不同作用时间其对AGS细胞迁移的抑制作用差异有统计学意义（P〈0.05）;同一时间不同浓度雷公藤红素对AGS细胞迁移的抑制作用差异有统计学意义（P〈0.05）。结论高浓度雷公藤红素能够使AGS细胞形态发生改变并凋亡;雷公藤红素抑制了AGS细胞的迁移,且该抑制作用与浓度、时间相关,且在终浓度为1μM作用于AGS细胞12 h时,其对AGS细胞迁移的抑制作用最明显。Objective To observe the effects of different concentration of celastrol on cellular mor-phology and migration of AGS cells at different time points;to explore a better inhibitory effect of concentra-tion and time of celastrol on migration of AGS cells. Methods AGS cells were planted and cultured in 6 -well plate. When the adherent cell density reached 70 ~ 80% ,cell migration was manufactured by scratch ex-periment. Thereafter,cell morphology and cell migration were observed under microscope with different con-centration of celastrol of 5 μM、1 μM、0. 5 μM、0. 1 μM、0. 01 μM at indicated time points. Results 1. High concentration of celastrol cause severe changed in the cell morphology. 2. Celastrol inhibited AGS cell migra-tion,and its inhibitory effect on the migration velocity was concentration - dependent in a certain range. The higher the concentration of drug,the stronger effect it was. The same concentration of celastrol on AGS cells at different times had different effect inhibition of cell migration（P ﹤ 0. 05）. At the same time the different con-centrations of celastrol on AGS cells had different effect inhibition of cell migration（P ﹤ 0. 05）. Conclusion 1. High concentrations of celastrol cause changes in the cell morphology and cell apoptosis. 2. Celastrol in-hibited AGS cell migration,which is dependent on the concentration and action time. The inhibitory effect of celastrol on AGS cell migration is most obvious under final concentration of 1 μM at 12 h.

关 键 词：雷公藤红素 细胞迁移 AGS细胞 不同浓度 不同时间 

分 类 号：R285.5[医药卫生—中药学]